Gorgonian and scleractinian coral t

Gorgonian and scleractinian coral tissue sample
decalcification
Gorgonian and scleractinian coral tissue samples were
transferred to a decalcifying solution (100g ethylenediamine
tetraacetic acid [EDTA] (Fisher Scientific, Waltham, MA), 10g
NaOH/L filtered seawater, pH 7.4) and placed in a LabPulse™
Microwave Tissue Processor (H 2850, Energy Beam Sciences,
CT). Sea fan fragments between 10 to 20mm2 were decalcified
for 20 min, while larger fans fragments (e.g.,

Hasil (Bahasa Indonesia) 1:

Gorgonian and scleractinian coral tissue sampledecalcificationGorgonian and scleractinian coral tissue samples weretransferred to a decalcifying solution (100g ethylenediaminetetraacetic acid [EDTA] (Fisher Scientific, Waltham, MA), 10gNaOH/L filtered seawater, pH 7.4) and placed in a LabPulse™Microwave Tissue Processor (H 2850, Energy Beam Sciences,CT). Sea fan fragments between 10 to 20mm2 were decalcifiedfor 20 min, while larger fans fragments (e.g., <20mm2) and theA. cervicornis fragment were decalcified for 40 min (Table 1).Decalcification was determined to be completed when nosclerites (calcium carbonate spines) were observed on thegorgonian sample surface, and the calcium carbonate skeletonwas dissolved from the scleractinian sample. Preliminaryexperiments were done using different power levels of themicrowave and reagent volumes to determine the optimalmicrowave settings for coral tissue (data not shown). Forthe results presented in this report, tissues were processedat 40oC for 20-40 min at a working power of 80% (720 watts).Note that the microwave power level for decalcification andtissue processing was set significantly higher (720 watts)than the power level that normally has been suggested(250-500 watts) [14]. The overall results suggested that atconstant temperature, low volume and a high power level(720 watts), the decalcification process and tissue processingwere accelerated without detrimentally affecting the integrityHistological processing using microwave technologyStep Solutions Vol.(ml) Temp(0c) Time(min)1 Decal-solution 300 40 20-402 Ethanol 95% 500 40 43 Ethanol 95%/100%2-Propanol500 40 44 100% 2-Propanol 500 40 45 2-Propanol/Paraffin 500 80 106 Paraffin vacuum -- 60 30Table 1. Steps, with volumes, temperatures, and times required forhistological processing using microwave technology.of the tissues. Therefore, the temperature is controlled by thevolume of the reagent, and independent of the power levelsetting of the microwave.

Hasil (Bahasa Indonesia) 2:

Gorgonian dan scleractinian karang jaringan sampel
dekalsifikasi
Gorgonian dan karang scleractinian sampel jaringan yang
ditransfer ke solusi decalcifying (100g etilendiamin
asam tetraacetic [EDTA] (Fisher Scientific, Waltham, MA), 10g
NaOH / L disaring air laut, pH 7.4) dan ditempatkan dalam LabPulse ™
Microwave Tissue Processor (H 2850, Energi Beam Ilmu,
CT). Fragmen penggemar laut antara 10 sampai 20mm2 yang dekalsifikasi
selama 20 menit, sementara fans besar fragmen (misalnya, <20mm2) dan
A. cervicornis fragmen yang dekalsifikasi selama 40 menit (Tabel 1).
dekalsifikasi bertekad akan selesai bila tidak ada
sclerites (duri kalsium karbonat) yang diamati pada
permukaan sampel gorgonian, dan kerangka kalsium karbonat
dibubarkan dari sampel scleractinian. Awal
percobaan dilakukan dengan menggunakan tingkat daya yang berbeda dari
microwave dan reagen volume untuk menentukan optimal
pengaturan microwave untuk jaringan karang (data tidak ditampilkan). Untuk
hasil yang disajikan dalam laporan ini, jaringan yang diproses
pada suhu 40oC selama 20-40 menit pada daya kerja 80% (720 watt).
Perhatikan bahwa tingkat daya microwave untuk dekalsifikasi dan
jaringan pengolahan didirikan secara signifikan lebih tinggi (720 watt)
dari tingkat daya yang biasanya telah disarankan
(250-500 watt) [14]. Hasil keseluruhan menunjukkan bahwa pada
suhu konstan, volume rendah dan tingkat daya tinggi
(720 watt), proses dekalsifikasi dan jaringan pengolahan
dipercepat tanpa detrimentally mempengaruhi integritas
pengolahan histologi menggunakan teknologi microwave
Langkah Solusi Vol. (ml) Temp (0c) Waktu (menit)
1 Decal-solusi 300 40 20-40
2 Etanol 95% 500 40 4
3 Etanol 95% / 100%
2-Propanol
500 40 4
4 100% 2-Propanol 500 40 4
5 2-Propanol / Paraffin 500 80 10
6 Parafin vakum - 60 30
Tabel 1. Langkah, dengan volume, suhu, dan waktu yang diperlukan untuk
proses histologis menggunakan teknologi microwave.
dari jaringan. Oleh karena itu, suhu dikontrol oleh
volume reagen, dan independen dari tingkat daya
pengaturan microwave.