To determine the influence of the e

To determine the influence of the enteropeptidase heavy chain on substrate recognition, secreted variants of recombinant proenteropeptidase were prepared, with or without the
heavy chain. Proenteropeptidase was shown to be a zymogen with extremely low intrinsic protease activity, and it was activated efficiently by trypsin. The presence of the heavy chain slightly inhibited proenteropeptidase activation and had almost no effect on the recognition of the small peptide substrate Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide. However, the heavy chain had profound effects on the recognition of macromolecular substrates (trypsinogen) and inhibitors (pancreatic trypsin inhibitor and soybean trypsin inhibitor).

Hasil (Bahasa Indonesia) 2:

Untuk mengetahui pengaruh rantai berat enteropeptidase pengakuan substrat, varian disekresikan dari proenteropeptidase rekombinan disiapkan, dengan atau tanpa
rantai berat. Proenteropeptidase terbukti menjadi zymogen dengan aktivitas protease intrinsik sangat rendah, dan itu diaktifkan efisien oleh tripsin. Kehadiran rantai berat sedikit terhambat aktivasi proenteropeptidase dan hampir tidak berpengaruh pada pengakuan dari peptida substrat kecil Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide. Namun, rantai berat memiliki efek mendalam pada pengakuan substrat makromolekul (tripsinogen) dan inhibitor (pankreas tripsin inhibitor dan kedelai tripsin inhibitor).