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Seven bacterial strains, namely B.
Seven bacterial strains, namely B. cereus WSBC 10530 (clinical
isolate), S. aureus ATCC 25923 (clinical isolate), L. monocytogenes
ŽM58 (IHM, Würzburg, Germany), S. Infantis ŽM9 (poultry meat
isolate), E. coli O157:H7 ŽMJ 129 (clinical isolate), C. jejuni ATCC 33560
(bovine faeces isolate) and C. coli ATCC 33559 (pig faeces isolate),
were used for antibacterial testing. The cultivation/assay medium for
L. monocytogenes and E. coli O157:H7 was Tryptone Soy Broth or Agar
(TSB, TSA, Oxoid, Hampshire, UK), for B. cereus, S. aureus and S. Infantis
it was Müeller Hinton Broth or Agar (MHB, MHA, Oxoid, Hampshire,
UK), while for C. jejuni and C. coli defibrinated horse blood 5% (Oxoid,
Hampshire, UK) was added to MHB or MHA. Bacterial cultures for
antimicrobial testing were prepared by picking colony from 24-h-old
TSA/MHA plates and it was suspended in an appropriate medium
(5 mL). Cultures were grown aerobically for 20 h and continuously
shaken at 100 rpm at 37 °C, while Campylobacter spp. were grown
microaerobically at 42 °C. For antibacterial activity assays 1 mL of each
culture was diluted with TSB or MHB medium to 105–106 CFU/mL.
isolate), S. aureus ATCC 25923 (clinical isolate), L. monocytogenes
ŽM58 (IHM, Würzburg, Germany), S. Infantis ŽM9 (poultry meat
isolate), E. coli O157:H7 ŽMJ 129 (clinical isolate), C. jejuni ATCC 33560
(bovine faeces isolate) and C. coli ATCC 33559 (pig faeces isolate),
were used for antibacterial testing. The cultivation/assay medium for
L. monocytogenes and E. coli O157:H7 was Tryptone Soy Broth or Agar
(TSB, TSA, Oxoid, Hampshire, UK), for B. cereus, S. aureus and S. Infantis
it was Müeller Hinton Broth or Agar (MHB, MHA, Oxoid, Hampshire,
UK), while for C. jejuni and C. coli defibrinated horse blood 5% (Oxoid,
Hampshire, UK) was added to MHB or MHA. Bacterial cultures for
antimicrobial testing were prepared by picking colony from 24-h-old
TSA/MHA plates and it was suspended in an appropriate medium
(5 mL). Cultures were grown aerobically for 20 h and continuously
shaken at 100 rpm at 37 °C, while Campylobacter spp. were grown
microaerobically at 42 °C. For antibacterial activity assays 1 mL of each
culture was diluted with TSB or MHB medium to 105–106 CFU/mL.
0/5000
Seven bacterial strains, namely B. cereus WSBC 10530 (clinicalisolate), S. aureus ATCC 25923 (clinical isolate), L. monocytogenesŽM58 (IHM, Würzburg, Germany), S. Infantis ŽM9 (poultry meatisolate), E. coli O157:H7 ŽMJ 129 (clinical isolate), C. jejuni ATCC 33560(bovine faeces isolate) and C. coli ATCC 33559 (pig faeces isolate),were used for antibacterial testing. The cultivation/assay medium forL. monocytogenes and E. coli O157:H7 was Tryptone Soy Broth or Agar(TSB, TSA, Oxoid, Hampshire, UK), for B. cereus, S. aureus and S. Infantisit was Müeller Hinton Broth or Agar (MHB, MHA, Oxoid, Hampshire,UK), while for C. jejuni and C. coli defibrinated horse blood 5% (Oxoid,Hampshire, UK) was added to MHB or MHA. Bacterial cultures forantimicrobial testing were prepared by picking colony from 24-h-oldTSA/MHA plates and it was suspended in an appropriate medium(5 mL). Cultures were grown aerobically for 20 h and continuouslyshaken at 100 rpm at 37 °C, while Campylobacter spp. were grownmicroaerobically at 42 °C. For antibacterial activity assays 1 mL of eachculture was diluted with TSB or MHB medium to 105–106 CFU/mL.
Sedang diterjemahkan, harap tunggu..
Tujuh strain bakteri, yaitu B. cereus WSBC 10530 (klinis
isolat), S. aureus ATCC 25923 (isolat klinis), L. monocytogenes
ŽM58 (IHM, Würzburg, Jerman), S. infantis ŽM9 (daging unggas
isolat), E. coli O157: H7 ŽMJ 129 (isolat klinis), C. jejuni ATCC 33560
(feses sapi mengisolasi) dan C. coli ATCC 33559 (feses babi mengisolasi),
yang digunakan untuk pengujian antibakteri. Budidaya / assay media untuk
L. monocytogenes dan E. coli O157: H7 adalah Tryptone Soy Broth atau Agar
(TSB, TSA, Oxoid, Hampshire, UK), untuk B. cereus, S. aureus dan S. infantis
itu Mueller Hinton Broth atau Agar (MHB, MHA, Oxoid, Hampshire,
UK), sedangkan untuk C. jejuni dan C. coli defibrinated darah kuda 5% (Oxoid,
Hampshire, UK) ditambahkan ke MHB atau MHA. Kultur bakteri untuk
pengujian antimikroba disusun dengan memilih koloni dari 24 h berusia
piring TSA / MHA dan itu ditangguhkan di media yang tepat
(5 mL). Budaya ditumbuhkan aerobik selama 20 jam dan terus
terguncang pada 100 rpm pada 37 ° C, sementara Campylobacter spp. ditumbuhkan
microaerobically pada 42 ° C. Untuk tes aktivitas antibakteri 1 mL dari masing-masing
budaya diencerkan dengan TSB atau MHB menengah ke 105-106 CFU / mL.
isolat), S. aureus ATCC 25923 (isolat klinis), L. monocytogenes
ŽM58 (IHM, Würzburg, Jerman), S. infantis ŽM9 (daging unggas
isolat), E. coli O157: H7 ŽMJ 129 (isolat klinis), C. jejuni ATCC 33560
(feses sapi mengisolasi) dan C. coli ATCC 33559 (feses babi mengisolasi),
yang digunakan untuk pengujian antibakteri. Budidaya / assay media untuk
L. monocytogenes dan E. coli O157: H7 adalah Tryptone Soy Broth atau Agar
(TSB, TSA, Oxoid, Hampshire, UK), untuk B. cereus, S. aureus dan S. infantis
itu Mueller Hinton Broth atau Agar (MHB, MHA, Oxoid, Hampshire,
UK), sedangkan untuk C. jejuni dan C. coli defibrinated darah kuda 5% (Oxoid,
Hampshire, UK) ditambahkan ke MHB atau MHA. Kultur bakteri untuk
pengujian antimikroba disusun dengan memilih koloni dari 24 h berusia
piring TSA / MHA dan itu ditangguhkan di media yang tepat
(5 mL). Budaya ditumbuhkan aerobik selama 20 jam dan terus
terguncang pada 100 rpm pada 37 ° C, sementara Campylobacter spp. ditumbuhkan
microaerobically pada 42 ° C. Untuk tes aktivitas antibakteri 1 mL dari masing-masing
budaya diencerkan dengan TSB atau MHB menengah ke 105-106 CFU / mL.
Sedang diterjemahkan, harap tunggu..
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