Sectioning and hematoxylin and eosi

Sectioning and hematoxylin and eosin (H&E) staining
Thin sections (4-5μm) from gorgonian and scleractinian
coral tissue were obtained using a rotary microtome (model
820; American Optical, Buffalo, NY). Sections were affixed to
subbed-glass slides (chrome alum-gelatin coating solution:
50.0 g gelatin (Mallinckrodt, St. Louis, MO) and 5.0 g chromium
potassium sulfate (Mallinckrodt, St. Louis, MO) in 1 L distilled
deionized water.
Sections were deparaffinized with Histo-Clear twice, (30 s
each, National Diagnostics, Charlotte, NC), re-hydrated through
a descending series of ethanols (95%-70% ethanol each for 30
s), washed in distilled deionized water (2X each 30 s), stained
in Harris hematoxylin (C.I. #75290, Sigma Aldrich, St. Louis,
MO) solution for 1 min, washed in running tap water for 1
min, differentiated in Scott solution (10g sodium bicarbonate;
2g magnesium sulfate in 1 L distilled deionized water; [15])
for 30 s, and washed in running tap water again for 1 min.
Bluing was done using 0.2% ammonia water for 30 s to 1 min.
Sections were washed in running tap water for 5 min; rinsed in
95% alcohol (10 dips); counterstained in eosin Y (C.I. #45380,
National Aniline and Chemical Company, Buffalo, NY) solution
for 30 sec to 1 min; dehydrated through 95% alcohol and 2
changes of absolute alcohol, 2 min each; cleared in Histo-Clear
(2X each for 5 min); and coverslipped using Permount (VWR,
Radnor, PA), a xylene based mounting medium.

Hasil (Bahasa Indonesia) 2:

Sectioning dan hematoxylin dan eosin (H & E) pewarnaan
bagian Tipis (4-5μm) dari gorgonian dan scleractinian
jaringan karang diperoleh dengan menggunakan mikrotom putar (Model
820; Amerika Optical, Buffalo, NY). Bagian yang ditempelkan
slide subbed-kaca (krom alum-gelatin lapisan solusi:
50,0 g gelatin (Mallinckrodt, St. Louis, MO) dan 5,0 g kromium
kalium sulfat (Mallinckrodt, St. Louis, MO) dalam 1 L suling
air deionisasi.
Bagian yang deparaffinized dengan Histo-jelas dua kali, (30 s
masing-masing, Diagnostik Nasional, Charlotte, NC), kembali terhidrasi melalui
serangkaian turun dari ethanols (95% -70% etanol masing-masing untuk 30
s), dicuci di air suling deionisasi ( 2X setiap 30 s), bernoda
di Harris hematoxylin (CI # 75290, Sigma Aldrich, St. Louis,
MO) solusi untuk 1 menit, dicuci dalam menjalankan air keran selama 1
menit, dibedakan dalam larutan Scott (10g natrium bikarbonat;
2g magnesium sulfat dalam 1 L air suling deionisasi; [15])
. selama 30 s, dan dicuci dalam air keran lagi selama 1 menit
kebiruan dilakukan dengan menggunakan air amonia 0,2% selama 30 s untuk 1 menit.
Bagian dicuci dalam menjalankan air keran selama 5 min, dibilas di
95% alkohol (10 dips); counterstained di Eosin Y (CI # 45380,
anilina Nasional dan Chemical Company, Buffalo, NY) solusi
untuk 30 detik untuk 1 menit; dehidrasi melalui 95% alkohol dan 2
perubahan alkohol absolut, 2 menit setiap; dibersihkan di Histo-jelas
(2X masing-masing untuk 5 menit); dan coverslipped menggunakan Permount (VWR,
Radnor, PA), sebuah xylene berdasarkan medium mounting.