Stem cuttings of pointed gourd, obt

Stem cuttings of pointed gourd, obtained from Bihar
Agricultural College, Sabour, were planted in well
prepared field at University Department of Botany, T.
M. Bhagalpur University, Bhagalpur. Since it is a
dioecious crop, plants of both the sexes were planted
in the field.
Pollen germination studies were carried out in
different concentration of sucrose alone, in combination
with 0.01% boric acid, in surface culture and also in
Brewbaker and Kwack’s medium (Brewbaker & Kwack
1963, Shivanna & Rangaswamy 1992). Pollen grains,
which had germinated and produced pollen tubes in the
medium, were recorded. Length of pollen tube in
different concentrations was recorded 45 min after the
commencement of germination and percentage of pollen germination was calculated and the average length of
pollen tube was recorded.
2,3,5- triphenyl tetrazolium chloride (TTC) was
prepared in sucrose solution (0.5% TTC). A drop of
TTC solution was taken on a microslide. Small amount
of pollen grains were suspended and slide was covered
with a cover glass. Micro slides were incubated in dark
for 60 minutes. After incubation period, pollen grains
were observed under a microscope and pollen grains,
which turned red, were counted as viable.
For FCR test, pollen grains were mounted in
fluorescein diacetate (FDA) solution. Stock solution of
FDA was prepared in acetone (2mg/l). Calcium nitrate
(300mg/l) was added in sucrose solution (6%) to prevent
bursting of pollen grains. Five milliliter of sucrose
solution was taken in a small glass vial and drops of
stock solution of F. D.A. were added in the mixture till
it showed persistent turbidity. A drop of sucrose and
FDA mixture was taken on a microslide. Sufficient
amount of pollen grains were suspended and microslides
were kept in a humidity chamber (> 90% RH) for 5
to10 minutes. After the incubation period, pollen grains
were covered with a coverslip and were observed under
fluorescence microscope. Those pollen grains that
fluoresce brightly were taken as viable.

Hasil (Bahasa Indonesia) 2:

Stek batang labu menunjuk, yang diperoleh dari Bihar
Agricultural College, Sabour, ditanam di dalam sumur
lapangan disiapkan di Universitas Departemen Botani, T.
M. Bhagalpur University, Bhagalpur. Karena itu adalah
tanaman dioecious, tanaman dari kedua jenis kelamin ditanam
di lapangan.
Studi Pollen perkecambahan dilakukan dalam
konsentrasi yang berbeda dari sukrosa saja, dalam kombinasi
dengan asam borat 0,01%, dalam budaya permukaan dan juga di
media Brewbaker dan Kwack itu ( Brewbaker & Kwack
1963, Shivanna & Rangaswamy 1992). Serbuk sari,
yang telah berkecambah dan menghasilkan tabung serbuk sari di
media, dicatat. Panjang tabung serbuk sari dalam
konsentrasi yang berbeda tercatat 45 menit setelah
dimulainya perkecambahan dan persentase perkecambahan serbuk sari dihitung dan panjang rata-rata
tabung polen tercatat.
2,3,5- trifenil tetrazolium klorida (TTC) telah
disiapkan dalam larutan sukrosa (0,5% TTC). Setetes
larutan TTC diambil pada microslide a. Jumlah kecil
dari serbuk sari dihentikan dan geser ditutupi
dengan kaca penutup. Slide mikro diinkubasi dalam gelap
selama 60 menit. Setelah masa inkubasi, serbuk sari
diamati di bawah mikroskop dan serbuk sari biji-bijian,
yang berubah merah, yang dihitung sebagai layak.
Untuk tes FCR, serbuk sari yang dipasang di
diasetat fluorescein (FDA) solusi. Larutan stok
FDA dipersiapkan dalam aseton (2mg / l). Kalsium nitrat
(300mg / l) ditambahkan dalam larutan sukrosa (6%) untuk mencegah
pecahnya serbuk sari. Lima mililiter sukrosa
solusi diambil dalam botol kaca kecil dan tetes
larutan stok dari FDA ditambahkan dalam campuran sampai
itu menunjukkan kekeruhan persisten. Setetes sukrosa dan
campuran FDA diambil pada microslide a. Cukup
jumlah serbuk sari dihentikan dan microslides
disimpan dalam kelembaban ruang (> 90% RH) selama 5
sampai 10 menit. Setelah masa inkubasi, serbuk sari
ditutupi dengan kaca penutup dan diamati di bawah
mikroskop fluoresensi. Mereka serbuk sari yang
berpendar terang diambil sebagai layak.