Trypsin-activated HL-BEK had an app

Trypsin-activated HL-BEK had an apparent mass of 140 kDa under nonreducing conditions (Fig. 2, fifth lane), similar to that of uncleaved pro-HL-BEK (fourth lane), and contained two disulfide-linked fragments of 133 and 43 kDa (second and third lanes). Compared with the unreduced trypsin-digested sample (Fig. 2, fifth lane), the 133-kDa heavy chain (third lane) stained relatively faintly, suggesting that the heavy chain may be sensitive to degradation by trypsin. The apparent mass of activated L-BEK was also similar to that of uncleaved pro-LBEK (Fig. 2, eighth and ninth lanes), and upon reduction, L-BEK contained a 43-kDa fragment similar to that of HL-BEK (third lane). The predicted amino-terminal activation fragment of L-BEK therefore appears to be disulfide-linked to the 43-kDa fragment under nonreducing conditions. Amino acid sequencing of the 43-kDa fragment from either HL-BEK or L-BEK gave the sequence Ile-Val-Gly-Gly-Ser-Asp-Ser-Arg-Glu-Gly, indicating that cleavage occurred at the site predicted from the cDNA sequence, after Lys-800 of full-length BEK (5). No amino-
terminal sequence could be obtained for pro-L-BEK, pro-HL-BEK, or the 133-kDa chain of HL-BEK, suggesting that the amino terminus of the heavy chain is blocked in these
preparations.

Hasil (Bahasa Indonesia) 2:

Tripsin-diaktifkan HL-BEK memiliki massa jelas 140 kDa bawah nonreducing kondisi (2, jalur kelima Gambar.), Mirip dengan uncleaved pro-HL-BEK (jalur keempat), dan berisi dua fragmen disulfida-linked dari 133 dan 43 kDa (kedua dan jalur ketiga). Dibandingkan dengan tereduksi tripsin-dicerna sampel (Gambar. 2, jalur kelima), 133-kDa berat rantai (jalur ketiga) bernoda relatif sedikit, menunjukkan bahwa rantai berat mungkin sensitif terhadap degradasi oleh tripsin. Massa jelas diaktifkan L-BEK juga mirip dengan uncleaved pro-LBEK (Gambar. 2, kedelapan dan jalur kesembilan), dan setelah pengurangan, L-BEK berisi fragmen 43-kDa mirip dengan HL-BEK ( jalur ketiga). Amino-terminal fragmen aktivasi prediksi L-BEK karena itu tampaknya disulfida-terkait dengan fragmen 43-kDa dalam kondisi nonreducing. Asam amino sequencing dari fragmen 43-kDa baik dari HL-BEK atau L-BEK memberi urutan Ile-Val-Gly-Gly-Ser-Asp-Ser-Arg-Glu-Gly, yang menunjukkan bahwa pembelahan terjadi di situs diprediksi dari urutan cDNA, setelah Lys-800 dari full-length BEK (5). Tidak ada amino
urutan terminal dapat diperoleh untuk pro-L-BEK, pro-HL-BEK, atau rantai 133-kDa dari HL-BEK, menunjukkan bahwa ujung amino dari rantai berat diblokir di ini
persiapan.