Results and discussionAntibiotic se

Results and discussion
Antibiotic sensitivity of C. glycerinogenes
Yeasts isolated from nature are often more
resistant to many drugs and chemical substances
than laboratory strains (Chand-Goyal et al. 1999).
We assessed the sensitivity of C. glycerinogenes to
phleomycin (phleomycin), G418 (geneticin) and
hygromycin B. Suspensions were prepared from
log-phase growing cells and plated at a concentration
of 106
/ml on YPD plates containing different
concentrations of phleomycin, G418 (Amresco,
Solon, OH, USA), and hygromycin B (Roche, Basel,
Switzerland). Phleomycin (100 mg/ml) completely
inhibited growth, whereas some resistant colonies
appeared on plates containing 500 mg/mL of G418
and hygromycin B. Furthermore, growth of
C. glycerinogenes was also inhibited in liquid
cultures containing 50 mg/ml phleomycin. Therefore,
150 mg/ml phleomycin was selected as a
suitable concentration for screening of plasmids
conferring resistance in transformants.

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Hasil dan diskusiAntibiotik kepekaan C. glycerinogenesRagi yang terisolasi dari alam yang sering lebihtahan terhadap banyak obat dan bahan kimiadari laboratorium strain (Chand Goyal et al. 1999).Kami dinilai kepekaan C. glycerinogenes untukphleomycin (phleomycin), G418 (geneticin) danhygromycin B. suspensi disusun darilog-fase tumbuh sel-sel dan berlapis pada konsentrasidari 106/ml pada YPD piring berisi berbedakonsentrasi phleomycin, G418 (Amresco,Solon, OH, USA), dan hygromycin B (Roche, Basel,Swiss). Phleomycin (100 mg/ml) benar-benarmenghambat pertumbuhan, sedangkan beberapa koloni tahanmuncul di piring berisi 500 mg/mL G418dan hygromycin B. Selain itu, pertumbuhanC. glycerinogenes juga menghambat dalam cairanbudaya yang mengandung 50 mg/ml phleomycin. Oleh karena itu,phleomycin 150 mg/ml dipilih sebagaikonsentrasi cocok untuk skrining plasmidPenganugerahan perlawanan di transformants.