Image acquisitionTissue sections we

Image acquisition
Tissue sections were observed using appropriate filter sets for
Alexa 546, phalloidin 488, and TO-PRO-3® (642/661nm) in a
Nikon Eclipse 800 (E800) optical microscope equipped with
the modular Nikon C1 Plus laser scanning confocal system
that is fully controlled by Nikon Elements AR (NISO) software.
The E800 is equipped with differential interference contrast
(DIC) and 10X Plan Fluor (N.A.=0.30), 20X Plan Fluor (N.A.=0.45),
40X Plan Fluor (N.A.=0.65), 60X S Fluor with correction collar
ring (N.A.=0.85), and 100X Plan Apo Oil immersed (N.A.=1.40)
objectives. Images were acquired with a QImaging Retiga Exi
camera (QImaging, Canada) with color filter. Image calibration
and measurements were performed using Nikon NIS-Elements.
Adobe Photoshop CS4 was used for figure plate preparation

Hasil (Bahasa Indonesia) 1:

Akuisisi gambarBagian jaringan diamati menggunakan sesuai filter set untukAlexa 546, phalloidin 488 dan TO-PRO-3 ® (642/661nm) diMikroskop optik Nikon Eclipse 800 (E800) yang dilengkapi denganmodular Nikon C1 Plus laser confocal sistem pemindaianyang sepenuhnya dikendalikan oleh perangkat lunak Nikon elemen AR (NISO).E800 dilengkapi dengan gangguan diferensial kontras(DIC) dan 10 X Fluor rencana (N.A.=0.30), 20 X rencana Fluor (N.A.=0.45),40 x Fluor rencana (N.A.=0.65), 60 X S Fluor dengan koreksi kerahcincin (N.A.=0.85), dan 100 X rencana Apo minyak tenggelam (N.A.=1.40)tujuan. Gambar diperoleh dengan QImaging Retiga Exikamera (QImaging, Kanada) dengan filter warna. Gambar kalibrasidan pengukuran dilakukan menggunakan Nikon NIS-elemen.Adobe Photoshop CS4 digunakan untuk persiapan plat gambar