While, there is similarity and diff

While, there is similarity and differences between
our work to those of others, who immunized their
mice with whole bacteria for having hybridoma
generation (Pengsuk et al., 2010), but all of them give
the idea of possible use of monoclonal antibodies as
diagnostic tools in V.Cholerae. They claimed to
produce mouse monoclonal antibodies against
V.Cholerae, then conjugated to gold nanoparticle and
used it as immunochromatic strip test for direct
detection of V. cholerae O139 in seafood samples.
Relative to above study, Pengsuk et al. (2013) also
developed a strip test for the detection of V.Cholerae
O139 using monoclonal antibodies which specifically
bind to the lipopolysaccharide and capsular polysaccharide of
V. cholerae O139. While in this
work we used recombinant OMPw for both
immunization of mice and also screening of
hybridoma cells for having monoclonal antibodies. In
contrast to our work Pengsuk et al. (2010) immunized
their mice with five isolates of V. cholerae(VC 1, 5,
9, 13 and 14) fixed in 4% Formaldehyde.