Aim of chromatography
• The compounds of interest has to be separated from matrix components
• The signal to noise ratio must be as high
as possible
• The time consumption of analysis must be short
Mobile Phase / Stationary Phase
Mobile phase
Stationary
phase
Strong Weak
• A site in mobile phase and a stationary phase make contact via an interface that is set up.
• The affinity with the mobile phase and stationary phase varies with the solute
• Separation occurs due to differences in the speed of motion.
Chromato
-graphy / -graph / -gram / -grapher
• Chromatography: Analytical technique
• Chromatograph: Instrument
• Chromatogram: Obtained “picture”
• Chromatographer: Person
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