In the preceding equations, [E]o is

In the preceding equations, [E]o is total enzyme concentration, [E] is free enzyme concentration, [E.S] is enzyme-substrate
concentration, [S]o is initial substrate concentration, [S] is substrate concentration at any time
t,
and Km = (
k
2 +
k
-1)/
k
-1 for
Equation 9 and (
k
-1 +
k
2)
k
3/
k
1 (
k
2 +
k
3) for Equation 11.
What is the order of an enzyme-catalyzed reaction and how does one determine the order? Since [E]o is constant throughout the
reaction, assuming it is stable, the order of an enzyme-catalyzed reaction is determined by the relationship between [S]o and
K
m
(as long as [S]o >> [E]o; see assumption 2). When [S] o

Hasil (Bahasa Indonesia) 1:

In the preceding equations, [E]o is total enzyme concentration, [E] is free enzyme concentration, [E.S] is enzyme-substrate
concentration, [S]o is initial substrate concentration, [S] is substrate concentration at any time
t,
and Km = (
k
2 +
k
-1)/
k
-1 for
Equation 9 and (
k
-1 +
k
2)
k
3/
k
1 (
k
2 +
k
3) for Equation 11.
What is the order of an enzyme-catalyzed reaction and how does one determine the order? Since [E]o is constant throughout the
reaction, assuming it is stable, the order of an enzyme-catalyzed reaction is determined by the relationship between [S]o and
K
m
(as long as [S]o >> [E]o; see assumption 2). When [S] o<0.01
K
m, the order of the reaction is first order with respect to [S]o.
This can be readily seen from Equation 9. When [S]o <0.01
K
m, [S]o in the denominator can be ignored and
n
o=
V
max[S]o/
K
m
,(
n
o=
k´
[S]o), where
k´
=
V
max/
K
m, showing direct dependence on [S]o. A plot of In([S]o/[S]) versus time gives a straight-line plot
over several half-lives

Hasil (Bahasa Indonesia) 2:

Dalam persamaan sebelumnya, [E] o adalah konsentrasi enzim total, [E] adalah konsentrasi enzim bebas, [ES] adalah enzim-substrat
konsentrasi, [S] o adalah konsentrasi substrat awal, [S] adalah konsentrasi substrat setiap saat
t ,
dan Km = (
k
+ 2
k
-1) /
k
-1 untuk
Persamaan 9 dan (
k
-1 +
k
2)
k
3 /
k
1 (
k
2 +
k
3) untuk Persamaan 11.
Apa urutan dari enzim-katalis reaksi dan bagaimana cara menentukan urutan? Sejak [E] o konstan sepanjang
reaksi, dengan asumsi itu adalah stabil, urutan reaksi enzim-dikatalisasi ditentukan oleh hubungan antara [S] o dan
K
m
(selama [S] o >> [E] o, lihat asumsi 2). Ketika [S] o <0,01
K
m, urutan reaksi adalah orde pertama terhadap [S] o.
Hal ini dapat dengan mudah dilihat dari Persamaan 9. Ketika [S] o <0,01
K
m, [S] o di penyebut dapat diabaikan dan
n
o =
V
max [S] o /
K
m
, (
n
= o
k'
[S] o), di mana
k'
=
V
max /
K
m, menunjukkan ketergantungan langsung pada [S] o. Sebuah plot In ([S] o / [S]) terhadap waktu memberikan plot garis lurus
selama beberapa waktu paruh