seeds are aperients, diuretic tonic

seeds are aperients, diuretic tonic and febrifuge. Bark is antibiotic, astringent and febrifuge.
The main objectives of the study are to analysis the total phenolic and flavonoid content, reducing power, antioxidant activity.
METHODS AND MATERIALS Chemicals used
All chemicals were purchased from Sigma Chemical, Merck Chemical Supplies, SD fine and Himedia. All other chemicals used were obtained commercially and were of analytical grade.
Collection of plant material
Artocarpus heterophyllus (Jackfruit) and Manilkara zapota (Sapodilla) were collected from Pallamudir, Coimbatore, Tamil Nadu, India.
Preparation of Extracts
The seeds were shade dried for one month and coarsely powdered. Extraction of the active ingredient from the seed powder was carried out using specific method15. 25g of the powdered seeds were extracted by soxhlet apparatus using 250ml of four different solvent (ethanol, acetone, ethylacetate and water) in a separate flask. The extraction lasted for six hours. The extract obtained was concentrated by evaporation using water bath at 100°C and stored at 4°C in cold room.
Determination of Total Phenolic content
Total phenolic content was determined by Folin-Ciocalteu reagent16. 10ml of the samples were taken in test tubes and made up to the volume of 1ml with distilled water. Then 0.5ml of Folin-Ciocalteu phenol reagent (1:1 with water) and 2.5ml of sodium carbonate solution (20%) were added sequentially in each tube in triplicate manner. Soon after vortexing the reaction mixture, the test tubes were placed in dark for 40 minutes and the absorbance was recorded at 725nm against a reagent blank. All tests were carried out in triplicate. Total content of phenolic compounds in extracts was expressed as Gallic Acid Equivalent (GAE) in milligram per gram extract.
Determination of Total Flavonoid content
Flavonoid content was determined according to the aluminum chloride colorimetric method with some modifications17. The sample solution (0.5ml) was mixed with 1.5ml of 95% ethanol, 0.1ml of 10% aluminum chloride hexahydrate, 0.1ml of 1M potassium acetate, and

Hasil (Bahasa Indonesia) 1:

biji yang aperients, tonik diuretik dan obat penurun panas. Kulit antibiotik, zat, dan obat penurun panas.Tujuan utama studi akan analisis total fenolik dan kandungan flavonoid, mengurangi kekuasaan, aktivitas antioksidan.METODE dan bahan kimia yang digunakanSemua bahan kimia yang dibeli dari Sigma kimia, bahan kimia Merck, SD halus dan Himedia. Semua kimia lainnya digunakan diperoleh secara komersial dan yang analitis kelas.Koleksi bahan tanamanArtocarpus heterophyllus (nangka) dan sawo Manila (sawo) dikumpulkan dari Pallamudir, Coimbatore, Tamil Nadu, India.Persiapan ekstrakBenih yang teduh kering selama satu bulan dan bubuk kasar. Ekstraksi bahan aktif dari bubuk biji dilaksanakan menggunakan method15 tertentu. 25 gram bubuk biji diekstraksi oleh aparat soxhlet menggunakan 250ml pelarut berbeda empat (etanol, aseton, ethylacetate dan air) dalam labu terpisah. Ekstraksi berlangsung selama enam jam. Ekstrak yang diperoleh terkumpul oleh penguapan menggunakan mandi air pada 100° C dan disimpan pada 4° C di kamar dingin.Penentuan Total fenolik kontenJumlah fenolik konten ditentukan oleh reagent16 Folin-Ciocalteu. 10ml sampel diambil dalam tabung dan membuat untuk volume 1ml dengan air suling. Kemudian 0.5ml pereaksi fenol Folin-Ciocalteu (1:1 dengan air) dan 2,5 ml larutan natrium karbonat (20%) ditambahkan secara berurutan dalam setiap tabung dalam cara triplicate. Segera setelah vortexing campuran reaksi, tabung ditempatkan dalam gelap selama 40 menit dan absorbansi tercatat pada 725nm melawan reagen kosong. Semua tes dilakukan dalam rangkap tiga. Jumlah konten senyawa fenolik di ekstrak dinyatakan sebagai setara asam galat (GAE) dalam milligram per gram ekstrak.Penentuan kandungan Total FlavonoidKandungan flavonoid ditentukan berdasarkan aluminium klorida colorimetric metode dengan beberapa modifications17. Solusi sampel (0.5 ml) dicampur dengan 1.5 ml 95% etanol, 0, 1 ml 10% aluminium klorida hexahydrate, 0, 1 ml 1 M kalium asetat, dan