32.6 mol%, and 61.8 mol%, respectiv

32.6 mol%, and 61.8 mol%, respectively, after 96 h of reaction.
Meanwhile, for trials in which vacuum was applied after 9 h from
the start of reaction, at 102 h, the incorporation of n3 PUFA was
35.8 mol%, but the yield of PC was 45.8 mol%.
4. Conclusion
PC from soybean was successfully modified by incorporation of
residues of n3 PUFA derived from fish oil using immobilized
PLA1. Water content, temperature and enzyme loading were
shown to have significant effects on both the incorporation of
n3 PUFA into PC and yield of PC. Reaction conditions were taken
into consideration in terms of an appropriate balance between the
extent of incorporation of n3 PUFA residues and the yield of PC.
Although a slight decrease in n3 PUFA incorporation occurred,
vacuum application was highly effective for increasing the yield
of PC.