Dionex Corporation, USA, GP 50 grad

Dionex Corporation, USA, GP 50 gradient pump having LC
2
25 chromatography oven was used in ion chromatographic
method. Column used was Ion Pac AS4A-Sc Dionex having
4mm i.d. x 250 mm with precolumn AG 4A-Sc. Electrochemical
suppressor used was ASRS ultra II 4mm. The detector was a
conductometric detector ED 40. Samples were introduced into
the analytical column by direct injection with a sample loop.
The sample injection volume was 10 µL having flow rate of
1.5 µL/min. Eluent used was a mixture of NaHCO
in the concentration range of 1.7x10
-3
3
and Na
M and 1.8x10
M
respectively. The amount of chloride in authentic thiamine
hydrochloride sample was estimated by making calibration
curve in the range of 1x10
-4
& 1x10
M.
In cyclic voltammetric method, a silver disk was used as
-5
working electrode, saturated calomel as reference electrode
and a cylindrical platinum as counter electrode immersed into
a mixture of tetraethyl ammonium tetrafluoroborate (TEATFB)
and thiamine hydrochloride (vitamin B
). The amount of
chloride in thiamine hydrochloride (vitamin B
1
) was estimated
by using a calibration curve for chloride ion. A blank
calibration curve was obtained in the range of 1 mM to 50
mM HCl. For estimating the amount of chloride in thiamine
hydrochloride (vitamin B
1
1
), above mentioned HCl solution
was added into thiamine hydrochloride (vitamin B
) sample
solution. Cyclic voltammogram was recorded from 500 to 500
mV at a scan rate of 100 mV/sec. All
measurements were
carried
out at 25
C in argon environment.

Hasil (Bahasa Indonesia) 2:

Dionex Corporation, USA, GP 50 pompa gradien memiliki LC
2
25 kromatografi oven digunakan dalam kromatografi ion
metode. Kolom yang digunakan adalah Ion Pac AS4A-Sc Dionex memiliki
4mm id x 250 mm dengan precolumn AG 4A-Sc. Elektrokimia
penekan yang digunakan adalah ASRS II 4mm Ultra. Detektor adalah
ED detektor conductometric 40. Sampel diperkenalkan ke
kolom analitis dengan injeksi langsung dengan loop sampel.
Volume injeksi sampel adalah 10 uL memiliki laju aliran
1,5 uL / min. Eluen yang digunakan adalah campuran NaHCO
di kisaran konsentrasi 1.7x10
-3
3
dan Na
M dan 1.8x10
M
masing-masing. Jumlah klorida dalam otentik tiamin
sampel hidroklorida diperkirakan dengan membuat kalibrasi
kurva di kisaran 1x10
-4
& 1x10
M.
Dalam metode voltametri siklik, disk perak digunakan sebagai
-5
elektroda kerja, jenuh kalomel sebagai elektroda referensi
dan silinder platinum sebagai elektroda counter terbenam ke
campuran tetraethyl amonium tetrafluoroborate (TEATFB)
dan tiamin hidroklorida (vitamin B
). Jumlah
klorida di tiamin hidroklorida (vitamin B
1
) diperkirakan
dengan menggunakan kurva kalibrasi untuk ion klorida. Sebuah kosong
kurva kalibrasi diperoleh di kisaran 1 mM sampai 50
mM HCl. Untuk memperkirakan jumlah klorida di tiamin
hidroklorida (vitamin B
1
1
), yang disebutkan di atas solusi HCl
ditambahkan ke dalam tiamin hidroklorida (vitamin B
) sampel
solusi. Voltamogram siklik tercatat 500-500
mV pada tingkat scan 100 mV / detik. Semua
pengukuran
dilakukan
pada 25
C dalam lingkungan argon.