They also reported that the initial

They also reported that the initial screening was
done by dot blotting against a mixture of the five
isolates of V.cholerae used for immunization. The interesting point is that,
they screened their positive
cultures by dot blotting and western immunoassay against each of the 5
isolates of V. cholerae that were
used for the immunization of mice before cell fusion(Pengsuk
et al., 2011).

In order to determine whether the antigen which
recognized by each monoclonal antibodies in their
studies, was a protein or Lipopolysaccharide (LPS),
they incubated the used nitrocellulose strip in
proteinase K (Roche, Basel, Switzerland) at 1 unit mL −1
in Tris/HCl buffer (20 mM, pH8) containing 1mM CaCl2 for 1 h at room
temperature before subjected to monoclonal antibody. In our experiment
anti recombinant OMPw monoclonal and polyclonal
antibodies reacted with whole V. Cholerae
but not other related bacteria demonstrating the possibility of
using anti rOMPw antibodies as a diagnostic tool for
detection of V. Cholerae.