Gorgonian and scleractinian coral t

Gorgonian and scleractinian coral tissue sample
processing
Following decalcification, gorgonian and scleractinian coral
tissue samples were washed in filtered seawater twice (10
min each). Then, tissues were placed in the microwave for
dehydration in a sequence of 95% ethanol (Fisher Scientific,
Waltham, MA, 95% ethanol: 100% isopropanol (2-propanol
hereafter) [Fisher Scientific, Waltham, MA], and cleared in 100%
2-propanol. Each step lasted 4 min and temperature was set
to 40oC. Next, tissue samples were kept in the microwave for
infiltration in a 1:1 solution of melted paraffin and 2-propanol
under vacuum for 10 min at 60oC (Table 1). A second round of
infiltration was performed with 100% melted paraffin under
vacuum for 30 min at 60oC. During the infiltration process, a
sheet of PolarHeat™ (Energy Beam Sciences, CT) was used to
convert microwave energy to heat, resulting in an increase in
diffusivity of the paraffin through the tissue [14].

Hasil (Bahasa Indonesia) 1:

Sampel jaringan karang gorgonian dan scleractinianpengolahanBerikut karang dekalsifikasi, gorgonian dan scleractiniansampel jaringan itu dicuci di disaring air dua kali (10menit masing-masing). Kemudian, Jaringan ditempatkan dalam microwave selamadehidrasi dalam urutan yang 95% etanol (Fisher ilmiah,Waltham, MA, 95% etanol: 100% isopropanol (2-propanolakhirat) [Fisher Scientific, Waltham, MA], dan dihapus dalam 100%2-propanol. Setiap langkah yang berlangsung selama 4 menit dan suhu ditetapkanuntuk 40oC. Selanjutnya, sampel jaringan dipelihara dalam microwave selamainfiltrasi dalam larutan 1:1 parafin meleleh dan 2-propanoldi bawah vakum untuk 10 min di 60oC (Tabel 1). Putaran keduainfiltrasi dilakukan dengan parafin 100% meleleh di bawahvakum selama 30 menit di 60oC. Selama proses infiltrasi,lembar PolarHeat ™ (energi sinar ilmu, CT) digunakan untukmengubah microwave energi panas, mengakibatkan peningkatandiffusivity parafin melalui jaringan [14].