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Mature cashews were selected by first eliminatingall damaged and spoiled ones and especially those inwhich the process of fermentation had alreadystarted. The cashews were washed by submergingthem in chlorine water for about 30 minutes in orderto remove dirt and microorganisms and were finallywashed with running water to remove the residualchlorine. Upon weighing the washed whole cashewsthe peduncle was separated and weighed. Thepeduncle juice was obtained by crushing theseparated peduncle and filtering it through a cottoncloth. ºBrix of the integral peduncle juice was thendetermined. The ºBrix values were used to calculatesugar concentrations during alcoholic fermentation.To purify the juice, 3mL of gelatine (10%) per mL ofjuice were added so that the must pectin could beremoved. Ammonium sulphate and potassiumphosphate as nutrients and potassium metabisulfiteas disinfectant were also added. In order to obtain acashew wine with an alcohol concentration higherthan 100 g/L, sugar was added to the peduncle juicechaptalization, to obtain an initial concentration of120 g/L. Therefore, 30 g/L of sugar was added at thebeginning of fermentation and thereafter 170 g/L,when the concentration of saccharose in the mediumfell to 10 g/L. In this way the sucrose inhibition wasreduced. Alcoholic fermentation was started byinoculation of the must through the addition of 20g/Lof Fleischmann TM Sacharomyces cerevisiea generalbakers yeast. Fermentation was interrupted when thesubstrate concentration level fell to less than 20g/L,which characterizes a dry wine and is appropriate forvinegar production. The concentrations in g/L ofsubstrate (S), product (P), biomass (X) and total acidas well as pH were monitored for a period of 48hours of alcoholic fermentation. A vacuum filtrationwas performed to remove microorganisms. Thefiltered wine was poured into pasteurized dark greenglass bottles and was stocked for use in theproduction of cashew wine vinegar.
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