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As response variables, the capacity factor of lamivudine (k1), the resolution between tenofovir and efavirenz (Rs2, 3), and the retention time of efavirenz (tR3) were chosen. All experiments were performed in randomized order to minimize the effects of uncontrolled variables that may introduce a bias on the measurements. For an experimental design with three factors, the model including linear, quadratic, and cross terms can be expressed as:
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