There are many studies related to i

There are many studies related to immunological and
molecular methods for diagnosis of
Vibrio cholera
(
V.
cholerae
). However, most assays dependent on enrichment of
culture of bacteria, which need more time and
involves the use of costly equipment and reagents.
In this study Balb/c mice were immunized with
recombinant Outer Membrane Protein (rOMPw) of
vibrio cholerae
and splenocytes of hyper immunized
mice were fused with murine myeloma Sp2/0 cells. Positive hybridomas were selected by ELISA using
rOMPw as coating antigen. The monoclonal antibodies
from ascitic fluids were purified and its reaction
with rOMPw was assessed by ELISA. Polyclonal antibo
dies were also produced by immunization of rabbits
with the above mentioned antigen. The rabbit sera w
as affinity purified using Hi-Trap protein G column
.
The result showed that monoclonal antibody specific
to rOMPw has been successfully generated. The
monoclonal antibody reacted with recombinant OMPw i
n ELISA and immunonoblat method. Rabbit
polyclonal antibody was also bound to rOMPw by ELIS
A. The results of agglutination test with whole
bacteria also showed that both mouse monoclonal and
rabbit polyclonal antibodies reacted with
whole
vibrio cholera
but not other related
bacteria
. The purpose of this study was to check out if ant
i OMPw
antibodies could use as diagnostic assay for detect
ion of
V. cholerae
. Our results demonstrated that anti
recombinant OMPw monoclonal and polyclonal antibodi
es are able to diagnose whole bacteria in pure
culture using agglutination test but not by home ma
de immunochromatic strip test.

Hasil (Bahasa Indonesia) 1:

Ada banyak penelitian yang berkaitan dengan imunologi danmolekul metode untuk diagnosisKolera bacillus(V.cholerae). Namun, paling tes tergantung pada pengayaanbudaya bakteri, yang memerlukan waktu danmelibatkan penggunaan peralatan mahal dan reagen.Dalam studi ini Balb c tikus diimunisasi denganrekombinan luar membran Protein (rOMPw)Vibrio choleraedan splenocytes dari hyper diimunisasitikus yang menyatu dengan sel-sel Sp2 0 tumore myeloma. Positif hybridomas dipilih oleh ELISA menggunakanrOMPw sebagai lapisan antigen. Antibodi monoklonaldari ascitic cairan ditahirkan dan reaksi yangdengan rOMPw dinilai oleh ELISA. Poliklonal antibomati juga diproduksi oleh imunisasi kelincidengan di atas disebutkan antigen. Kelinci sera wsebagai afinitas dimurnikan menggunakan Hi-perangkap protein G kolom.Hasilnya menunjukkan bahwa monoclonal antibodi spesifikuntuk rOMPw telah berhasil dihasilkan. Theantibodi monoklonal bereaksi dengan rekombinan OMPwn metode ELISA dan immunonoblat. Kelinciantibodi poliklonal juga terikat untuk rOMPw oleh ELISA. hasil aglutinasi tes dengan keseluruhanbakteri juga menunjukkan bahwa keduanya mouse monoklonal danantibodi poliklonal kelinci bereaksi dengankeseluruhankolera bacillustapi tidak terkait lainnyabakteri. Tujuan dari penelitian ini adalah untuk memeriksa jika semutsaya OMPwantibodi bisa digunakan sebagai uji diagnostik untuk mendeteksiIon bagiV. cholerae. Hasil kami menunjukkan bahwa antirekombinan OMPw antibodi poliklonal dan monoklonales mampu mendiagnosis seluruh bakteri dalam murnibudaya menggunakan tes aglutinasi tetapi tidak oleh rumah made immunochromatic strip tes.