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Results and DiscussionMethod development and ANNCurrently, a wide variety of chromatographic stationaryphases, providing significantly different retention and selectivity,are commercially available and principally offer theopportunity to perform any separation. However, manycolumns present similar characteristics, which made the selectionof a proper chromatographic system difficult and problemdependent. The sharpness of a chromatographic peak was anindication of the quality of the chromatographic column andits efficacy. Small particle size and long column dimensionsgenerally increase column efficiency, which in turn increasessensitivity, analytical resolution, and speed, though other characteristicshad a greater effect on separation.Column performance was assessed first using the five sigma
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