Rabies virus is a single-strand, ne

Rabies virus is a single-strand, negative-sense RNA virus
of the family Rhabdoviridae. Its nonsegmented genomic
RNA acts as a template for both transcription and replication.
During the transcription process, a positive-strand
leader RNA and five monocistronic mRNAs are synthesized,
whereas during the RNA replication process, a fulllength,
positive-strand RNA (replicative intermediate) is synthesized,
which serves as the template for the synthesis of
progeny negative-strand genomic RNA (Wunner, 1991). The
progeny genomic RNA is encapsidated with nucleoprotein
(N) and this RNA–N complex, together with the nominal
viral phosphoprotein (P, also designated NS or M1) and
RNA polymerase (L), forms the active cytoplasmic ribonucleoprotein
(RNP) complex in virus-infected cells and the
RNP core in virus particles (Sokol et al., 1971). The RNP
complex alone is capable of initiating viral RNA transcription
and replication in vivo and in vitro. Recent studies of
rabies viral protein functions using reverse genetics demonstrated
that N, P, and L in the RNP complex are all
required for rabies virus RNA transcription and replication
(Conzelmann and Schnell 1994; Schnell et al., 1994). However,
the sequence of events, specificity, and mode(s) of
interaction between each of these proteins and between
these proteins and the genomic RNA which results in viral
RNA transcription and replication has not been defined

Hasil (Bahasa Indonesia) 1:

Rabies virus adalah virus RNA tunggal-strand, negatif-sensedari keluarga Rhabdoviridae. Yang nonsegmented genomRNA bertindak sebagai template untuk transkripsi dan replikasi.Selama proses transkripsi, positif-strandpemimpin RNA dan lima monocistronic mRNAs disintesis,Sedangkan selama proses replikasi RNA, fulllength,positif-strand RNA (replikasi menengah) disintesis,yang berfungsi sebagai template untuk sintesisketurunan negatif-strand genom RNA (Wunner, 1991). Theketurunan genom RNA yang encapsidated dengan nucleoprotein(N) dan kompleks RNA-N ini, bersama dengan nominalvirus phosphoprotein (P, juga ditunjuk NS atau M1) danRNA polimerase (L), membentuk ribonucleoprotein sitoplasma aktif(RNP) kompleks di sel-sel yang terinfeksi virus danRNP inti dalam partikel virus (Sokol et al., 1971). RNPkompleks saja mampu memulai virus RNA transkripsidan replikasi di vivo dan in-vitro di. Penelitian terbaru darifungsi protein virus rabies yang menggunakan reverse genetika menunjukkanitu N, P, dan L di kompleks RNP semuanyadiperlukan untuk rabies virus RNA transkripsi dan replikasi(Conzelmann dan Schnell 1994; Schnell et al, 1994). Namun,urutan peristiwa, kekhususan, dan mode permainan dariinteraksi antara masing-masing dari protein dan antaraprotein dan RNA genomik yang mengakibatkan virusTranskripsi RNA dan replikasi belum didefinisikan