A stochastic burst model for preQ1-

A stochastic burst model for preQ1-dependent expression of
the Tte mRNA. In the absence of ligand (top), a transcription event
(grey arrow) gives rise to an individual riboswitch-containing mRNA
molecule that persists in the cell until it is degraded (light red arrow). During
its lifetime, the mRNA transitions between burst (green bar segments) and
non-burst (red bar segments) conformational states during which its SD
sequence (purple nucleotides) is highly accessible and largely occluded,
respectively. As reflected by increased anti-SD probe binding during SiMKARTS,
there are more potential opportunities for translation initiation events
(blue arrows) in the burst state, leading to bursts of protein biosynthesis,
than in the non-burst state. In the presence of the ligand (bottom), preQ1
favours formation of the full P2 stem (green nucleotides) and occlusion
of the SD sequence, resulting in shorter excursions to the burst state and,
consequently, fewer opportunities for translation initiation. This may be
accentuated by a decrease in the mRNA’s lifetime (depicted as overall
shorter burst/non-burst bars) resulting from its scarce occupancy with
actively translating ribosomes, leading to significant downregulation of
protein expression

Hasil (Bahasa Indonesia) 1:

Stokastik meledak model untuk ekspresi bergantung pada preQ1Tte mRNA. Dalam ketiadaan ligan (atas), acara transkripsi(abu-abu panah) menimbulkan mRNA yang mengandung riboswitch individumolekul yang bertahan di sel sampai terdegradasi (panah merah terang). Selamaseumur hidup, mRNA transisi antara meledak (hijau bar segmen) danBebas-burst (merah bar segmen) konformasi Serikat selama SD yangurutan (ungu nukleotida) sangat mudah diakses dan sebagian besar tersumbat,masing-masing. Seperti yang tercermin oleh peningkatan anti-SD probe mengikat selama SiMKARTS,ada lebih banyak peluang untuk terjemahan inisiasi acara(biru panah) dalam keadaan meledak, menyebabkan ledakan sintesis protein,daripada di negara bebas-burst. Hadapan ligan (bawah), preQ1nikmat pembentukan P2 penuh batang (hijau nukleotida) dan oklusiurutan SD, mengakibatkan kunjungan singkat ke negara meledak dan,Akibatnya, lebih sedikit kesempatan untuk inisiasi terjemahan. Ini mungkinditekankan oleh penurunan mRNA seumur hidup (digambarkan sebagai keseluruhanpendek meledak/bebas-burst Bar) dihasilkan dari hunian yang langka denganaktif menerjemahkan ribosom, menyebabkan signifikan downregulation dariekspresi protein