Molecular Identification of Selecte

Molecular Identification of Selected Strains.
The strains that were selected based on their production
of acetic, lactic, and propionic acids were identified
as belonging to 3 different species: Lb. plantarum, Lb.
brevis, and Lb. hilgardii, with sequence identity >97%
(Table 3).
Experiment 2: Evaluation of Strains
in Experimental Silos
Chemical and Microbiological Characteristics
of Silage. The use of inoculated strains affected (P <
0.001) DM losses and NDF content in the sugar cane
silage (Table 4). In the silages inoculated with strains
UFLA SIL17, UFLA SIL24, UFLA SIL25, UFLA
SIL27, UFLA SIL33, UFLA SIL51, and UFLA SIL52,
lower DM losses were found compared with the control.
Inoculation with these strains also resulted in silage
with a higher DM content and lower NDF. The inoculants
did not influence (P = 0.91) pH or water-soluble
carbohydrates content of the silage, whose mean values
were of 3.61 and 25.9 g/kg of DM, respectively.
The populations of LAB (P < 0.001) and yeast (P =
0.02) differed among the silages (Table 4). All silages
inoculated with LAB strains showed a reduction in
LAB population compared with the control, except for
those inoculated with UFLA SIL51 and UFLA SIL52
strains. The yeast population was larger in the silage
inoculated with UFLA SIL19, UFLA SIL32, UFLA
SIL42, and UFLA SIL46 strains. Among the remaining
silage samples, the yeast population was smaller than
those previously reported.
The production of metabolites after 60 d of fermentation
was influenced (P < 0.01) by the addition of inoculants
(Table 5). The silage inoculated with the UFLA
SIL19, UFLA SIL32, UFLA SIL34, UFLA SIL35,
UFLA SIL41, UFLA SIL42, and UFLA SIL46 strains
showed high levels of lactic acid and ethanol, similar to
the control silage. The use of UFLA SIL51 and UFLA
SIL52 strains resulted in silage with similar levels of acetic acid and 1,2-propanediol, which were higher than
in the other silages. The acetic acid levels in the silage
inoculated with UFLA SIL17, UFLA SIL24, UFLA
SIL25, UFLA SIL27, UFLA SIL33, and UFLA SIL42
strains were similar and showed no difference from the
control silage. However, these acetic acid levels were
higher than those found for the silage inoculated with
the UFLA SIL19, UFLA SIL32, UFLA SIL34, UFLA
SIL35, UFLA SIL41, and UFLA SIL46 strains, which
showed the lowest acetic acid levels. The propionic
acid levels were higher in the silage inoculated with
the UFLA SIL17, UFLA SIL25, UFLA SIL27, UFLA
SIL32, UFLA SIL33, UFLA SIL34, UFLA SIL35, and
UFLA SIL41 strains than the other silages. For the other
silages, the propionic acid levels were low and similar,
consistent with the control silage. The concentrations the UFLA SIL19, UFLA SIL42, UFLA SIL46, UFLA
SIL51, and UFLA SIL52 strains, similar to the control
(P > 0.05). The other silage showed similar levels of
butyric acid.
Temperature of Silage After Opening the Silos.
We observed no differences (P > 0.05) among silages
in aerobic stability, maximum temperature reached by
the silage, or time to reach this maximum temperature
(Table 6). The average of the maximum temperature
reached by the silage ranged from 41.8°C (UFLA
SIL19) to 45.7°C (UFLA SIL17), and the time to reach
this maximum temperature varied from 29.3 h (UFLA
SIL24) to 53.3 h (UFLA SIL19, UFLA SIL42, and
UFLA SIL46). The temperature of the control silage
was stable for approximately 21.3 h, whereas that of
the silage produced with the inoculants was stable for
periods ranging from 18.7 to 29.3 h (Table 6).

Hasil (Bahasa Indonesia) 1:

Molekul identifikasi galur yang dipilih.Strain yang dipilih berdasarkan produksi merekaasetat, laktat, dan asam propionat diidentifikasisebagai milik 3 spesies yang berbeda: Lb. plantarum, Lb.Brevis, dan Lb. hilgardii, dengan urutan identitas > 97%(Tabel 3).Percobaan 2: Evaluasi galurdi eksperimental SilosKarakteristik kimia dan Mikrobiologidari silase. Penggunaan terlepas strain terpengaruh (P <0.001) DM kerugian dan NDF konten dalam tebusilase (Tabel 4). Dalam silages diinokulasi dengan strainUFLA SIL17, UFLA SIL24, UFLA SIL25, UFLASIL27, UFLA SIL33, UFLA SIL51, dan UFLA SIL52,lebih rendah kerugian DM ditemukan dibandingkan dengan kontrol.Inokulasi dengan galur tersebut juga mengakibatkan silasedengan DM tinggi konten dan menurunkan NDF. Inokulantidak mempengaruhi (P = 0.91) pH atau larut dalam airkandungan karbohidrat silase, nilai-nilai yang berartiyang 3,61 dan 25.9 g/kg DM, masing-masing.Populasi LAB (P < 0.001) dan ragi (P =0,02) berbeda antara silages (Tabel 4). Semua silagesdiinokulasi dengan strain laboratorium menunjukkan penurunanLAB populasi dibandingkan dengan kontrol, kecuali untukmereka diinokulasi dengan UFLA SIL51 dan UFLA SIL52strain. Populasi ragi itu lebih besar dalam silasediinokulasi dengan UFLA SIL19, UFLA SIL32, UFLASIL42, dan UFLA SIL46 strain. Antara sisasilase sampel, populasi ragi adalah lebih kecil daripadamereka dilaporkan sebelumnya.Produksi metabolit setelah 60 d fermentasidipengaruhi (P < 0.01) dengan penambahan inokulan(Tabel 5). Silase diinokulasi dengan UFLASIL19, UFLA SIL32, UFLA SIL34, UFLA SIL35,Strain UFLA SIL41, UFLA SIL42 dan UFLA SIL46menunjukkan tingkat tinggi asam laktat dan etanol, miripsilase kontrol. Penggunaan UFLA SIL51 dan UFLASIL52 strain mengakibatkan silase dengan tingkat yang sama dari asam asetat dan 1,2-propanediol, yang lebih tinggi daripadadalam silages lain. Kadar asam asetat dalam silasediinokulasi dengan UFLA SIL17, UFLA SIL24, UFLASIL25, UFLA SIL27, UFLA SIL33, dan UFLA SIL42strain serupa dan menunjukkan tidak ada perbedaan darikontrol silase. Namun, tingkat asam asetat ini adalahlebih tinggi daripada yang ditemukan untuk silase diinokulasi denganSIL19 UFLA, UFLA SIL32, UFLA SIL34, UFLASIL35, UFLA SIL41 dan UFLA SIL46 strain, yangmenunjukkan tingkat asam asetat yang terendah. Propionattingkat asam yang tinggi dalam silase diinokulasi denganSIL17 UFLA, UFLA SIL25, UFLA SIL27, UFLASIL32, UFLA SIL33, UFLA SIL34, UFLA SIL35, danUFLA SIL41 strain daripada silages lain. Untuk yang lainsilages, asam propionat tingkat yang rendah dan serupa,konsisten dengan kontrol silase. Konsentrasi SIL19 UFLA, UFLA SIL42, UFLA SIL46, UFLASIL51, dan UFLA SIL52 strain, mirip dengan kontrol(P > 0,05). Silase lainnya menunjukkan serupa tingkatasam butirat.Suhu silase setelah membuka Silos.Kami mengamati ada perbedaan (P > 0,05) antara silagesdalam stabilitas aerobik, suhu maksimum dicapai dengansilase, atau waktu untuk mencapai suhu maksimum ini(Tabel 6). Rata-rata suhu maksimumdicapai dengan silase berkisar dari 41,8 ° C (UFLASIL19) 45.7 ° C (UFLA SIL17), dan waktu untuk mencapaisuhu maksimum ini bervariasi dari 29.3 h (UFLASIL24) 53.3 h (UFLA SIL19, UFLA SIL42, danUFLA SIL46). Suhu kontrol silasestabil untuk sekitar 21.3 h, sedangkan yangsilase diproduksi dengan inokulan adalah stabil untukperiode mulai dari 18.7 29.3 h (Tabel 6).