2.8. HardnessTexture profile analys

2.8. Hardness
Texture profile analyses (TPA) of sea bassfillets were carried out
at room temperature using a TA-XT plus texture analyser (Stable
Micro Systems Ltd., Godalming, UK) equipped with a 50-mm
diameter cylindrical aluminum probe, which approached thefillet
at the speed of 2 mm/s. A penetration depth of 3 mm into the
sample was selected as the maximum distance that could be
applied without breaking the muscle fibers and affecting the
muscle structure by disrupting it and leaving a mark on the sample.
Three sampling points were selected on eachfillet [dorsal, tail
(8 mm from the edge of tail) and between dorsal and tail]. Thefillet
was allowed to rebound for 15 s with the compression plate just
touching the surface. Double compression was applied to construct
the TPA parameters. The compression plate was then pressed on the
fillet a second time and hardness was obtained by analyzing the
first force peak as described by Anton and Luciano (2007). The
obtained values were expressed as g/cm
2
. Three experiments were
made for each sample.
2.9. Color analysis
The surface color was measured at three points in the sea bass
fillet (anterior, middle, posterior), using a WSC-S colorimeter
(Shanghai Precision Instrument Co. Ltd., Shanghai, China). To
analyze theL*(black/white), a*(red/green) and b*(yellow/blue)
values, three measurements were taken for eachfillet.
2.10. Biogenic amines
BAs of all samples were determined according to the methods
described byPark et al. (2010). Briefly, 5 g of each sample were
homogenized with 20 ml 0.1 M hydrochloric acid using a homogenizer for 1 min. The homogenate was centrifuged at 11,190 g for
15 min, and the supernatant was collected. The residue was
extracted again with 20 ml 0.1 M hydrochloric acid. The supernatants were then combined and adjusted to 50 ml with 0.1 M hydrochloric acid. A stock of standard solution was prepared by
adding an accurately weighed amount of each amine (100 mg) to a
100 ml volumetricflask and brought to the mark with 0.1 M HCl.
The standard solutions were stored at 4

C until use. Each extracted
sample or standard solution (0.3 ml) was mixed with 0.05 ml of
saturated NaHCO3and 0.05 ml of 2 M NaOH. 0.3 ml of 10 mg/ml
DNS-Cl solution prepared in acetone was added and the reaction
mixture was incubated at 45
C for 1 h in darkness. Residual DNS-Cl
was removed by adding 0.02 ml 25% ammonia. After 30 min the
mixture was adjusted to 1.0 ml with acetonitrile and centrifuged at
2417gfor 10 min. The supernatant was filtered through 0.22-mm
filters prior to HPLC analysis.
The quantification of BAs was carried out using an optimized
reverse-phase HPLC (Agilent, 1260 LC) equipped with a Agilent C18
(5mm, 4.6250 mm) column and a UV detector (Agilent, 1260 LC).
Distilled water (solvent A) and acetonitrile (solvent B) were used as
mobile phases. Elution was carried out using the following
gradient: 0 min, 55% B; 15 min, 65% B; 20 min, 80% B; 30 min, 90% B;
35 min, 55% B. The sample (10ml) was injected at aflow rate of 1 ml/
min and the peaks were detected at 254 nm.

Hasil (Bahasa Indonesia) 1:

2.8. kekerasanTekstur profil analisis (TPA) laut bassfillets dilakukanpada suhu kamar menggunakan analyser TA-XT ditambah tekstur (stabilMicro Systems Ltd, Godalming, Inggris) dilengkapi dengan 50-mmdiameter silinder aluminium probe, yang mendekati thefilletpada kecepatan 4 mm/s. Kedalaman penetrasi 3 mm kesampel dipilih sebagai jarak maksimum yang dapatditerapkan tanpa melanggar Serabut otot dan mempengaruhistruktur otot dengan mengganggu itu dan meninggalkan tanda pada sample.Tiga poin sampling dipilih pada eachfillet [ekor dorsal,(8 mm dari ujung ekor) dan antara dorsal dan ekor]. Thefilletdiizinkan untuk rebound untuk 15 s dengan piring kompresi hanyamenyentuh permukaan. Double kompresi diterapkan untuk membangunparameter TPA. Kompresi piring kemudian ditekanfillet kedua kalinya dan kekerasan ini diperoleh dengan menganalisispertama memaksa puncak seperti yang dijelaskan oleh Anton dan Luciano (2007). Thenilai-nilai yang diperoleh diungkapkan sebagai g/cm2. Tiga eksperimendibuat untuk setiap sampel.2.9. analisis warnaWarna permukaan diukur pada tiga titik dalam bass lautfillet (posterior anterior, tengah,), menggunakan colorimeter WSC-S(Shanghai presisi instrumen Co Ltd, Shanghai, Cina). Untukmenganalisis theL*(black/white), a*(red/green) dan b*(yellow/blue)nilai-nilai, tiga pengukuran diambil untuk eachfillet.2.10. Biogenic aminaBAs semua sampel telah ditetapkan menurut metodedijelaskan byPark et al. (2010). Secara singkat, 5 g dari setiap sampel yanghomogen dengan 20 ml asam klorida 0.1 M menggunakan pengaduk magnet selama 1 menit. Homogenate disentrifugasi 11,190 g untuk15 menit, dan supernatant dikumpulkan. Residu adalahdiekstrak lagi dengan 20 ml asam klorida 0.1 M. Supernatants kemudian digabungkan dan disesuaikan dengan 50 ml dengan asam klorida 0.1 M. Saham larutan standar disiapkan olehmenambah jumlah yang akurat ditimbang setiap amina (100 mg)volumetricflask 100 ml dan dibawa ke tanda dengan 0.1 M HCl.Solusi standar disimpan di 4C sampai digunakan. Masing-masing yang diekstraksisampel atau standar solusi (0.3 ml) dicampur dengan 0.05 mljenuh NaHCO3and 0.05 ml 2 M NaOH. 0.3 ml 10 mg/mLSolusi DNS-Cl disiapkan di aseton ditambahkan dan reaksicampuran diinkubasi di 45C untuk 1 h dalam kegelapan. DNS-Cl sisadihapus dengan menambahkan 0,02 ml 25% amonia. Setelah 30 menitcampuran disesuaikan 1,0 ml dengan asetonitril dan disentrifugasi di2417gfor 10 menit. Supernatant disaring melalui 0.22-mmfilter sebelum HPLC analisis.Kuantifikasi BAs dilakukan menggunakan dioptimalkanSebaliknya-fase HPLC (Agilent, 1260 LC) dilengkapi dengan Agilent C18(5mm, 4.6 250 mm) kolom dan detektor UV (Agilent, 1260 LC).Air distilasi (pelarut A) dan asetonitril (pelarut B) digunakan sebagaiMobile tahap. Elution dilakukan menggunakan berikutgradien: 0 menit, 55% B; 15 min, 65% B; 20 min, 80% B; 30 min, 90% B;35 menit, 55% B. Sampel (10 mililiter) disuntikkan pada tingkat aflow 1 ml /min dan puncak-puncak yang terdeteksi di 254 nm.