LinearityPrecisionIntra-day precisi

Linearity
Precision
Intra-day precision was determined by performing a five replicates
of three concentration levels of standard alpha-mangostin on the
same day. Similar procedures were duplicated on two different days in
order to evaluate the inter-day (intermediate) precision. The results as
summarised in table 1 showed acceptable repeatability measurements
with relative standard deviation not exceeded 2%. It is worth noting
that the %RSD values of intermediate precision were relatively large
which suggest instability of alpha-mangostin in the solvent mixture of
RT= 7.02 ± 0.01
N= 22171.25 ± 216.68
H= 153.81 ± 0.34
7.021
A B
C D
E F
0.8
0.6
0.4
0.2
0
-0.2
0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14
0 2 4 6 8 10 12 14
0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14
Minutes Minutes
Minutes Minutes
Minutes Minutes
mAU
mAU mAU
mAU mAU
mAU
250
200
150
100
50
0
60
50
40
30
20
10
0
0.6
0.5
0.4
0.3
0.2
0.1
0
-0.1
300
250
200
150
100
50
0
140
120
100
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20
0
RT= 7.30 ± 0.40
N= 22878.48 ± 414.54
H= 279.73 ± 25.70
RT= 7.48 ± 0.04
N= 23927.98 ± 147.10
H= 303.10 ± 1.30
RT= 7.17 ± 0.01
N= 24183.05 ± 605.33
H= 6.27 ± 0.01
RT= 7.13 ± 0.12
N= 23389.69 ± 1051.45
H= 62.02 ±0.10
7.262 7.177
7.942
6.660
7.457
7.180
Figure 3: HPLC chromatograms of (A) Typical blank (solvent) baseline in which
the solvent peak appeared within the first two minutes followed by the unstable
meanders 8 min as the result of gradient elution and disappeared upon reconditioning
of the column; (B to D) were alpha-mangostin reference standards
of 0.2 μg/mL, 20 μg/mL and 100 μg/mL, respectively; (E) Own isolated, pure
alpha-mangostin 100 μg/ml and (F) Extracted alpha-mangostin from the PLGA
microspheres. The results of retention time (RT), number of theoretical plate (N)
and peak height (H) were expressed as the mean of three replicates ± standard
deviation.
The linearity of the method was found be linear within the range of
1-200 μg/ml with regression equation of “Y=37.5X+25.98” that showed
a good correlation coefficient of 0.9995. In addition, we had also plotted
lower range concentration of 0.2-0.8 μg/ml with regression equation
of “Y=38.507X-0.0333” and found the good correlation wa

Hasil (Bahasa Indonesia) 1:

LinearitasPresisiPresisi intra-hari ditentukan dengan melakukan lima bereplikasitiga tingkat konsentrasi alpha-mangostin standar padahari yang sama. Prosedur serupa digandakan didua hari berbeda diperintah untuk mengevaluasi presisi antar hari (menengah). Hasil sebagaidiringkas dalam tabel 1 menunjukkan diterima pengulangan pengukurandengan deviasi standar yang relatif tidak melebihi 2%. Perlu dicatatnilai RSD % menengah presisi yang relatif besaryang menunjukkan ketidakstabilan alpha-mangostin dalam campuran pelarutRT @ = 7.02 ± 0.01N = 22171.25 ± 216.68H = 153.81 ± 0,347.021BC DE F0,80.60.40.200,6730 2 4 6 8 10 12 14 0 2 4 6 8 10 12 140 2 4 6 8 10 12 140 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14Menit menitMenit menitMenit menitmAUmAU mAUmAU mAUmAU25020015010050060504030201000.60,50.40.30.20.100.1300250200150100500140120100806040200RT @ = 7.30 ± 0,40N = 22878.48 ± 414.54H = 279.73 ± 25.70RT @ = 7.48 ± 0,04N = 23927.98 ± 147.10H = 303.10 ± 1,30RT @ = 7.17 ± 0,01N = 24183.05 ± 605.33H = 6,27 ± 0,01RT @ = 7.13 ± 0.12N = 23389.69 ± 1051.45H = 62.02 ± 0.107.262 7.1777.9426.6607.4577.180Gambar 3: HPLC chromatograms () khas kosong (solvent) dasar di manapuncak pelarut muncul dalam dua menit pertama diikuti dengan tidak stabilliku 8 min sebagai hasil dari gradien elution dan menghilang setelah rekondisikolom; (B d) adalah alpha-mangostin referensi standar0.2 μg/mL, 20 μg/mL, dan 100 μg/mL, masing-masing; (E) sendiri terisolasi, murniAlpha-mangostin 100 μg/ml dan (F) Extracted alpha-mangostin dari PLGAmikrosfer. Hasil waktu retensi (RT), jumlah teori pelat (N)dan puncak tinggi (H) yang dinyatakan sebagai rata-rata tiga Ulangan ± standarpenyimpangan.Linearitas dari metode ditemukan menjadi linier dalam jangkauan1-200 μg/ml dengan persamaan regresi "Y = 37.5 X + 25.98" yang menunjukkanKoefisien korelasi yang baik dari 0.9995. Selain itu, kami juga telah diplotmenurunkan konsentrasi berkisar 0,2-0,8 μg/ml dengan persamaan regresidari "Y = 38.507 X-0.0333" dan menemukan korelasi yang baik wa