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Abstract: The selection of a DNA ex
Abstract: The selection of a DNA extraction method is a critical step when subsequent
analysis depends on the DNA quality and quantity. Unlike mammals, for which several
capable DNA extraction methods have been developed, for molluscs the availability of
optimized genomic DNA extraction protocols is clearly insufficient. Several aspects such
as animal physiology, the type (e.g., adductor muscle or gills) or quantity of tissue, can
explain the lack of efficiency (quality and yield) in molluscs genomic DNA extraction
procedure. In an attempt to overcome these aspects, this work describes an efficient
method for molluscs genomic DNA extraction that was tested in several species from
different orders: Veneridae, Ostreidae, Anomiidae, Cardiidae (Bivalvia) and Muricidae
(Gastropoda), with different weight sample tissues. The isolated DNA was of high
molecular weight with high yield and purity, even with reduced quantities of tissue.
Moreover, the genomic DNA isolated, demonstrated to be suitable for several downstream
molecular techniques, such as PCR sequencing among others.
analysis depends on the DNA quality and quantity. Unlike mammals, for which several
capable DNA extraction methods have been developed, for molluscs the availability of
optimized genomic DNA extraction protocols is clearly insufficient. Several aspects such
as animal physiology, the type (e.g., adductor muscle or gills) or quantity of tissue, can
explain the lack of efficiency (quality and yield) in molluscs genomic DNA extraction
procedure. In an attempt to overcome these aspects, this work describes an efficient
method for molluscs genomic DNA extraction that was tested in several species from
different orders: Veneridae, Ostreidae, Anomiidae, Cardiidae (Bivalvia) and Muricidae
(Gastropoda), with different weight sample tissues. The isolated DNA was of high
molecular weight with high yield and purity, even with reduced quantities of tissue.
Moreover, the genomic DNA isolated, demonstrated to be suitable for several downstream
molecular techniques, such as PCR sequencing among others.
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Abstract: The selection of a DNA extraction method is a critical step when subsequentanalysis depends on the DNA quality and quantity. Unlike mammals, for which severalcapable DNA extraction methods have been developed, for molluscs the availability ofoptimized genomic DNA extraction protocols is clearly insufficient. Several aspects suchas animal physiology, the type (e.g., adductor muscle or gills) or quantity of tissue, canexplain the lack of efficiency (quality and yield) in molluscs genomic DNA extractionprocedure. In an attempt to overcome these aspects, this work describes an efficientmethod for molluscs genomic DNA extraction that was tested in several species fromdifferent orders: Veneridae, Ostreidae, Anomiidae, Cardiidae (Bivalvia) and Muricidae(Gastropoda), with different weight sample tissues. The isolated DNA was of highmolecular weight with high yield and purity, even with reduced quantities of tissue.Moreover, the genomic DNA isolated, demonstrated to be suitable for several downstreammolecular techniques, such as PCR sequencing among others.
Sedang diterjemahkan, harap tunggu..
Abstrak: Pemilihan metode ekstraksi DNA merupakan langkah penting saat berikutnya
analisis tergantung pada kualitas dan kuantitas DNA. Tidak seperti mamalia, yang beberapa
metode ekstraksi DNA mampu telah dikembangkan, untuk moluska ketersediaan
protokol ekstraksi DNA genom dioptimalkan jelas tidak cukup. Beberapa aspek seperti
sebagai fisiologi hewan, jenis (misalnya, otot adduktor atau insang) atau kuantitas jaringan, dapat
menjelaskan kurangnya efisiensi (kualitas dan hasil) di moluska ekstraksi DNA genom
prosedur. Dalam upaya untuk mengatasi aspek-aspek ini, pekerjaan ini menjelaskan efisien
metode untuk moluska ekstraksi DNA genom yang diuji pada beberapa spesies dari
perintah yang berbeda: Veneridae, Ostreidae, Anomiidae, Cardiidae (Bivalvia) dan Muricidae
(Gastropoda), dengan jaringan sampel berat yang berbeda . DNA terisolasi adalah tinggi
berat molekul dengan hasil tinggi dan kemurnian, bahkan dengan mengurangi jumlah jaringan.
Selain itu, DNA genom diisolasi, terbukti cocok untuk beberapa hilir
teknik molekuler, seperti PCR sequencing antara lain.
analisis tergantung pada kualitas dan kuantitas DNA. Tidak seperti mamalia, yang beberapa
metode ekstraksi DNA mampu telah dikembangkan, untuk moluska ketersediaan
protokol ekstraksi DNA genom dioptimalkan jelas tidak cukup. Beberapa aspek seperti
sebagai fisiologi hewan, jenis (misalnya, otot adduktor atau insang) atau kuantitas jaringan, dapat
menjelaskan kurangnya efisiensi (kualitas dan hasil) di moluska ekstraksi DNA genom
prosedur. Dalam upaya untuk mengatasi aspek-aspek ini, pekerjaan ini menjelaskan efisien
metode untuk moluska ekstraksi DNA genom yang diuji pada beberapa spesies dari
perintah yang berbeda: Veneridae, Ostreidae, Anomiidae, Cardiidae (Bivalvia) dan Muricidae
(Gastropoda), dengan jaringan sampel berat yang berbeda . DNA terisolasi adalah tinggi
berat molekul dengan hasil tinggi dan kemurnian, bahkan dengan mengurangi jumlah jaringan.
Selain itu, DNA genom diisolasi, terbukti cocok untuk beberapa hilir
teknik molekuler, seperti PCR sequencing antara lain.
Sedang diterjemahkan, harap tunggu..
Bahasa lainnya
Dukungan alat penerjemahan: Afrikans, Albania, Amhara, Arab, Armenia, Azerbaijan, Bahasa Indonesia, Basque, Belanda, Belarussia, Bengali, Bosnia, Bulgaria, Burma, Cebuano, Ceko, Chichewa, China, Cina Tradisional, Denmark, Deteksi bahasa, Esperanto, Estonia, Farsi, Finlandia, Frisia, Gaelig, Gaelik Skotlandia, Galisia, Georgia, Gujarati, Hausa, Hawaii, Hindi, Hmong, Ibrani, Igbo, Inggris, Islan, Italia, Jawa, Jepang, Jerman, Kannada, Katala, Kazak, Khmer, Kinyarwanda, Kirghiz, Klingon, Korea, Korsika, Kreol Haiti, Kroat, Kurdi, Laos, Latin, Latvia, Lituania, Luksemburg, Magyar, Makedonia, Malagasi, Malayalam, Malta, Maori, Marathi, Melayu, Mongol, Nepal, Norsk, Odia (Oriya), Pashto, Polandia, Portugis, Prancis, Punjabi, Rumania, Rusia, Samoa, Serb, Sesotho, Shona, Sindhi, Sinhala, Slovakia, Slovenia, Somali, Spanyol, Sunda, Swahili, Swensk, Tagalog, Tajik, Tamil, Tatar, Telugu, Thai, Turki, Turkmen, Ukraina, Urdu, Uyghur, Uzbek, Vietnam, Wales, Xhosa, Yiddi, Yoruba, Yunani, Zulu, Bahasa terjemahan.
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