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[Salinan]Disalin!
Vmax varies with the total concentration of enzyme present.
Km is independent of enzyme concentration and is characteristic of the system.
Km values lie in the range 10-2 to 10-6 mol/l
Km indicates the relative “suitability” of alternate substrates of particular enzyme
Substrate with the lowest Km vaue has the highest affinity for the enzyme
The “best” substrate is that which has the highest Vmax/Km ratio
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