FecundityTo investigate fecundity a

Fecundity
To investigate fecundity and fertilization
success of mature zebrafish, spawning trays were placed into all control and test
tanks to induce spawning behavior. One hour
after the light had gone on in the morning;
spawned eggs were collected from the spawning
trays and counted every day. Fecundity
studies were carried out for both control and
experimental fishes prior to deltamethrin exposure
for 6 days and during exposure period
of six days. The number of fertilized and unfertilized
eggs were counted and recorded.
Blood sampling, plasma preparation and
Hormone analysis
After six days of exposure to DM,
blood was collected by anaesthetizing with
MS-222 (methanesulfonate salt; Sigma). Immediately
following anaesthesia, the caudal
peduncle was severed transversely, and the
blood was taken from the caudal artery/vein
with a heparinized microhematocrit capillary
tube. An equal volume of aprotinin buffer
(6μg/ml in phosphate buffered saline) was
added to the microcapillary tube, and plasma
was separated from the blood by centrifuging
at 1500rpm for 5 min. Following this capillary
tubes were freeze dried in liquid nitrogen and
stored at -80°C until further analysis. Hormones
namely vitellogenin, 17β estradiol and
11-ketotestosterone were analyzed using kits
from biosense laboratories, Norway (Cayman
chemicals company) by enzyme-linked immunosorbent
assay (ELISA) using μQuant
plate reader from Bio-Tek, USA. Protocol
supplied by the manufacturers was followed
separately for each hormone.
Collection of gonads for histological processing
After collection of blood and gonads
were removed from both male and female and
were fixed in bouin’s solution (0.9% picric acid,
9%formaldehyde and 5% acetic acid). Histological
processing was carried out following
the procedure given out by Nolan et al,
(2001). Briefly tissues are replaced with 70%
industrial methylated spirit (IMS). This process
was repeated several times to remove the
excess of picric acid and stored at 4°C. Later
they were dehydrated with increasing IMS
(70% 90% 95% and 100% respectively),
cleared with xylene and then embedded in
paraffin wax. They were cut using a rotary
microtome (type No.RM2125RTF, leica microsystems,
China) with disposable microtome
blades (Shandon, MB35) and subsequently
stained with haematoxylin and eosin (H&E).
Photomicrographs were taken using magnus
(MLX) equipment.
Statistical analysis
Data were statistically analyzed using
the DMR (Duncan’s multiple range) test.The
value P

Hasil (Bahasa Indonesia) 1:

FecundityTo investigate fecundity and fertilizationsuccess of mature zebrafish, spawning trays were placed into all control and testtanks to induce spawning behavior. One hourafter the light had gone on in the morning;spawned eggs were collected from the spawningtrays and counted every day. Fecunditystudies were carried out for both control andexperimental fishes prior to deltamethrin exposurefor 6 days and during exposure periodof six days. The number of fertilized and unfertilizedeggs were counted and recorded.Blood sampling, plasma preparation andHormone analysisAfter six days of exposure to DM,blood was collected by anaesthetizing withMS-222 (methanesulfonate salt; Sigma). Immediatelyfollowing anaesthesia, the caudalpeduncle was severed transversely, and theblood was taken from the caudal artery/veinwith a heparinized microhematocrit capillarytube. An equal volume of aprotinin buffer(6μg/ml in phosphate buffered saline) wasadded to the microcapillary tube, and plasmawas separated from the blood by centrifugingat 1500rpm for 5 min. Following this capillarytubes were freeze dried in liquid nitrogen andstored at -80°C until further analysis. Hormonesnamely vitellogenin, 17β estradiol and11-ketotestosterone were analyzed using kitsfrom biosense laboratories, Norway (Caymanchemicals company) by enzyme-linked immunosorbentassay (ELISA) using μQuantplate reader from Bio-Tek, USA. Protocolsupplied by the manufacturers was followedseparately for each hormone.Collection of gonads for histological processingAfter collection of blood and gonadswere removed from both male and female andwere fixed in bouin’s solution (0.9% picric acid,9%formaldehyde and 5% acetic acid). Histologicalprocessing was carried out followingthe procedure given out by Nolan et al,(2001). Briefly tissues are replaced with 70%industrial methylated spirit (IMS). This processwas repeated several times to remove theexcess of picric acid and stored at 4°C. Laterthey were dehydrated with increasing IMS(70% 90% 95% and 100% respectively),cleared with xylene and then embedded inparaffin wax. They were cut using a rotarymicrotome (type No.RM2125RTF, leica microsystems,China) with disposable microtomeblades (Shandon, MB35) and subsequentlystained with haematoxylin and eosin (H&E).Photomicrographs were taken using magnus(MLX) equipment.Statistical analysisData were statistically analyzed usingthe DMR (Duncan’s multiple range) test.Thevalue P<0.05 was used as the criterion for statisticalsignificance (Duncan, 1955). All dataare expressed as mean ± SD (n=8).

Hasil (Bahasa Indonesia) 2:

Fekunditas
Untuk menyelidiki fekunditas dan pemupukan
keberhasilan ikan zebra dewasa, pemijahan nampan ditempatkan ke semua kontrol dan uji
tank untuk mendorong perilaku pemijahan. Satu jam
setelah lampu telah pergi di pagi hari;
menelurkan telur yang dikumpulkan dari pemijahan
nampan dan dihitung setiap hari. Fekunditas
penelitian dilakukan untuk kedua kontrol dan
ikan eksperimental sebelum deltametrin eksposur
selama 6 hari dan selama periode paparan
enam hari. Jumlah dibuahi dan tidak dibuahi
telur dihitung dan dicatat.
pengambilan sampel darah, persiapan plasma dan
analisis hormon
Setelah enam hari dari paparan DM,
darah dikumpulkan oleh khasiat anestetik dengan
MS-222 (garam methanesulfonate; Sigma). Segera
setelah anestesi, yang ekor
batang diputuskan melintang, dan
darah diambil dari ekor arteri / vena
dengan mikrohematokrit kapiler heparinized
tabung. Volume yang sama dari penyangga aprotinin
(6μg / ml dalam fosfat buffer saline) telah
ditambahkan ke dalam tabung microcapillary, dan plasma
dipisahkan dari darah oleh sentrifugasi
pada 1500rpm selama 5 menit. Setelah kapiler ini
tabung yang beku-kering dalam nitrogen cair dan
disimpan pada -80 ° C sampai analisis lebih lanjut. Hormon
yaitu vitellogenin, 17β estradiol dan
11-ketotestosterone dianalisis dengan menggunakan kit
dari laboratorium biosense, Norwegia (Cayman
kimia perusahaan) oleh enzim-linked immunosorbent
assay (ELISA) menggunakan μQuant
pembaca piring dari Bio-Tek, USA. Protokol
yang disediakan oleh produsen diikuti
secara terpisah untuk masing-masing hormon.
Koleksi gonad untuk pengolahan histologis
Setelah pengumpulan darah dan organ reproduksi
telah dihapus dari laki-laki dan perempuan dan
difiksasi dalam larutan Bouin dunia (0,9% asam picric,
9% formalin dan 5% asetat AC id). Histologis
pengolahan dilakukan menyusul
prosedur yang diberikan oleh Nolan et al,
(2001). Jaringan singkat diganti dengan 70%
termetilasi semangat industri (IMS). Proses ini
diulang beberapa kali untuk menghilangkan
kelebihan asam picric dan disimpan pada suhu 4 ° C. Kemudian
mereka dehidrasi dengan meningkatnya IMS
(masing-masing 70% 90% 95% dan 100%),
dibersihkan dengan xylene dan kemudian tertanam dalam
lilin parafin. Mereka memotong menggunakan rotary
mikrotom (tipe No.RM2125RTF, leica Microsystems,
Cina) dengan pakai mikrotom
pisau (Shandon, MB35) dan kemudian
diwarnai dengan hematoksilin dan eosin (H & E).
Photomicrographs diambil menggunakan magnus
(MLX) peralatan.
Analisis statistik
Data dianalisis secara statistik menggunakan
DMR (multiple kisaran Duncan) test.The
nilai P <0,05 digunakan sebagai kriteria untuk statistik
signifikansi (Duncan, 1955). Semua data
dinyatakan sebagai mean ± SD (n = 8).