Total phenol concentration The tota

Total phenol concentration The total amount of phenols was determined by using Folin-Ciocalteu’s phenol reagent and spectrophotometric determination. Samples (200 mL, three replicates) were mixed with 1.0 mL Folin-Ciocalteu’s phenol reagent (diluted 1:10 with water) and 0.8 mL of a 7.5% (w/v) sodium carbonate solution was added. The reaction mixture was stored for 30 min. at room temperature before measuring the absorbance at 765 nm on a Cintra 40, UV-Visible spectrophotometer (GBC scientific equipment, USA).

Hasil (Bahasa Indonesia) 2:

Konsentrasi total fenol Jumlah total fenol ditentukan dengan menggunakan Folin Ciocalteu-itu fenol reagen dan tekad spektrofotometri. Sampel (200 mL, tiga ulangan) dicampur dengan 1,0 mL Folin Ciocalteu-itu fenol reagen (diencerkan 1:10 dengan air) dan 0,8 mL dari 7,5% (b / v) larutan natrium karbonat ditambahkan. Campuran reaksi disimpan selama 30 menit. pada suhu kamar sebelum mengukur absorbansi pada 765 nm pada Cintra 40, UV-Visible spektrofotometer (GBC peralatan ilmiah, USA).