preserved and readily defined at lo

preserved and readily defined at low (Figure 3a) and high
magnification (Figures 3b and 3c). An epithelium ranging in
thickness from 29 to 37μm is clearly observed separating the
external environment from the gastrodermal tissue (Figures 3a
and 3c). Within the gastrodermal tissue, round zooxanthellae
ranging from 5 to 7μm in diameter are surrounded by vacuoles
within host cells (Figure 3b). In the mesoglea, sclerite openings
are shown, surrounded by sclerocytes with round basophilic
positive nuclei ranging in diameter from 3 to 6μm (Figure 3c).
The new protocol is also useful for describing the cytology
of diseased tissues. For instance, the characteristic features
of diseased sea fan tissue: a complete absence of epithelium,
broken mesoglea, and gastrodermal channels with their
zooxanthellae outside their host gastrodermal cells are readily
observed (Figure 4a). Hyphae–like structures surrounded by
skeletal material as well as capsules, spores, and filamentouslike
structures are also observed within the diseased tissue
(Figures 4b and 4d).
Additionally, the use of the accelerated microwave procedure
assures the antigenicity of proteins, enabling us to perform
fluorescence immunohistochemistry. For instance, Figure 5 shows immunohistochemistry staining of a healthy sea fan
tissue. Filaments of actin are primarily detected on the axial
skeleton, whereas Aspergillus spp. is detected across the
coenenchyme, bordering the polyps, and the axial skeleton.
Nuclei are detected throughout the cells found across
coenenchyme and polyps. Moreover, this result adds further
evidence supporting the argument that fungi and in particular
Aspergillus fungi are common to healthy sea fans [19-22].
As with gorgonians, this technique can also be used, with
excellent results, on scleractians coral. For instance, Figure 6
shows tentacle epithelium of a healthy A. cervicornis. Numerous
spirocyst across the epithelia are observed, while junction
of mesoglea and myoepithelial cells are seemingly right
underneath the epithelium.

Hasil (Bahasa Indonesia) 1:

diawetkan dan mudah didefinisikan di rendah (Gambar 3a) dan tinggipembesaran (angka 3b dan 3c). Epitel mulaiketebalan dari 29 untuk 37μm dengan jelas diamati memisahkanlingkungan eksternal dari jaringan gastrodermal (angka 3adan 3 c). Dalam jaringan gastrodermal, putaran zooxanthellaemulai dari 5 hingga 7μm diameter dikelilingi oleh vakuoladalam sel host (angka ayat 3b). Di mesoglea, sclerite bukaanditampilkan, dikelilingi oleh sclerocytes dengan putaran basophilicpositif inti mulai diameter dari 3 6μm (gambar 3c).Protokol baru ini juga berguna untuk menggambarkan sitologidari jaringan yang sakit. Misalnya, fitur karakteristikJaringan berpenyakit kipas laut: absen lengkap dari epitel,patah mesoglea, dan gastrodermal saluran dengan merekazooxanthellae di luar sel-sel gastrodermal tuan rumah mereka adalah mudahdiamati (Gambar 4a). Hyphae-seperti struktur dikelilingi olehbahan rangka serta kapsul, spora, dan filamentouslikestruktur juga diamati dalam jaringan berpenyakit(Angka 4b dan 4d).Selain itu, penggunaan akselerasi microwave prosedurmenjamin antigenisitas protein, memungkinkan kita untuk melakukanfluoresensi immunohistochemistry. Misalnya, Gambar 5 menunjukkan pewarnaan immunohistochemistry penggemar laut yang sehatjaringan. Filamen mikrovili terutama terdeteksi pada aksialkerangka, sedangkan Aspergillus spp. terdeteksi di seluruhcoenenchyme, berbatasan dengan polip, dan kerangka aksial.Inti terdeteksi seluruh sel-sel yang ditemukan di seluruhcoenenchyme dan polip. Selain itu, hasil ini menambahkan lebih lanjutbukti yang mendukung argumen bahwa jamur dan khususnyaAspergillus jamur umum untuk sehat laut penggemar [19-22].Seperti dengan gorgonians, teknik ini juga dapat digunakan, denganhasil yang sangat baik, pada scleractians karang. Misalnya, gambar 6menunjukkan tentakel epitel cervicornis A. sehat. Banyakspirocyst di epithelia yang diamati, sementara junctionmesoglea dan mioepitel sel-sel tampaknya tepatdi bawah epitel.