AbstractBackground: Microwave techn

Abstract
Background: Microwave technology has revolutionized histological processing by reducing processing
time and improving tissue integrity. We describe the first microwave-accelerated tissue processing
procedure for healthy and diseased tissue fragments of two species of corals: the gorgonian Gorgonia
ventalina and the scleractinian Acropora cervicornis.
Methods: Fourteen tissue samples from sea fans (eleven healthy and three diseased), and one tissue sample
from a healthy A. cervicornis, were decalcified and processed using microwaves. Histological slices were
stained using hematoxylin and eosin and immunostained using an antibody against Aspergillus.
Results: By using microwave technology, the decalcification time, as well as the steps and time for tissue
processing were significantly reduced while maintaining the integrity of the tissue.
Conclusions: This technique accelerates the chemical processing of coral tissue, while providing high
quality and optimal resolution of histological sections.
Keywords: Histology, microwave, tissue processing, coral diseases

Hasil (Bahasa Indonesia) 1:

AbstrakLatar belakang: Microwave teknologi telah merevolusi histologis pengolahan dengan mengurangi pengolahanwaktu dan meningkatkan integritas jaringan. Kami menjelaskan proses jaringan microwave-accelerated pertamaprosedur untuk jaringan sehat dan sakit fragmen dua spesies karang: gorgonian Gorgoniaventalina dan scleractinian Acropora cervicornis.Metode: Empat belas sampel jaringan dari laut penggemar (sebelas sehat dan tiga berpenyakit), dan satu jaringan sampeldari sehat A. cervicornis, decalcified dan diproses dengan menggunakan microwave. Irisan histologis yangpatri menggunakan hematoxylin dan eosin dan menggunakan antibodi terhadap Aspergillus immunostained.Hasil: dengan menggunakan teknologi microwave, saat dekalsifikasi, serta langkah-langkah dan waktu untuk jaringanpengolahan secara signifikan dikurangi sambil mempertahankan integritas dari jaringan.Kesimpulan: Teknik ini mempercepat proses kimia karang jaringan, sementara menyediakan tinggikualitas dan resolusi optimal histologis bagian.Kata kunci: Histologi, microwave, Jaringan pengolahan, karang penyakit