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The present study was approved by the Animal ExperimentalCommittee of Obihiro University of Agriculture and VeterinaryMedicine, in accordance with the Guiding Principles for the Careand Use of Research Animals.AnimalsThe present experiment was conducted at Shibetsu Sheep Farmin Hokkaido, Japan, during the non-breeding season (July 2007). Atotal of 104 mature Suffolk ewes (2–9 years old) were used. Basically,the ewes were fed 3 kg/day of hay (mainly orchards)supplemented with 300 g/day of concentrates (13% crude proteinand 76% total digestible nutrients) and were provided with freeaccess to fresh water and mineral blocks throughout the study.
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