Antimicrobial activity of stingless

Antimicrobial activity of stingless bee (Trigona sp.) propolis used in the folk medicine of Western Maharashtra, India
Ethnopharmacological relevance: Stingless bee (Trigona sp.) propolis is widely used in the folk medicine of Western Maharashtra, India to treat a variety of ailments.
Aim of the study: To determine the chemical composition and evaluate the antimicrobial activity of Indian stingless bee propolis.
Materials and methods: Chemical composition of the ethanolic extract of propolis (EEP) was determined by GC–MS analysis. A range of bacteria including multidrug resistant (MDR) cultures as well as a yeast strain was tested for antimicrobial activity using EEP. MIC, MBC, MFC, Kill curves and post agent effect (PAE) of the EEP were assessed using standard microbiological methods.
Results: GC–MS analysis revealed that propolis contained 24 compounds (9 known and 15 newly reported). Many of these were known bioactive compounds, including antimicrobials. The MICs of EEP were in the range of 1.21–9.75 g/mL while the MBCs/MFC were between 2.43 and 19.5 g/mL. The time required to achieve 90% (1 log10 ) reduction in culture growth ranged between 1.06 and 3.53 h at their respective MIC values. PAE for all the cultures was >24 h.

Conclusion: Indian stingless bee propolis has a complex nature with 24 chemical compounds. It has a potent broad-spectrum antimicrobial activity. The latter finding, in conjunction with other bioactive properties, could provide a scientific basis to its popular use in the Indian folk medicine.

Hasil (Bahasa Indonesia) 2:

Aktivitas antimikroba Lebah (Trigona sp.) Propolis digunakan dalam obat rakyat dari Barat Maharashtra, India
ethnopharmacological relevansi: lebah bersengat (. Trigona sp) propolis banyak digunakan dalam obat rakyat dari Barat Maharashtra, India untuk mengobati berbagai penyakit .
Tujuan dari penelitian ini: Untuk menentukan komposisi kimia dan mengevaluasi aktivitas antimikroba stingless propolis lebah India.
Bahan dan metode: komposisi kimia dari ekstrak etanol propolis (EEP) ditentukan dengan analisis GC-MS. Berbagai bakteri termasuk multidrug resistant (MDR) budaya serta strain ragi diuji aktivitas antimikroba menggunakan EEP. MIC, MBC, MFC, Bunuh kurva dan posting efek agen (PAE) dari EEP dinilai menggunakan metode mikrobiologi standar.
Hasil: analisis GC-MS mengungkapkan bahwa propolis mengandung 24 senyawa (9 dikenal dan 15 baru dilaporkan). Banyak dari mereka yang dikenal senyawa bioaktif, termasuk antimikroba. The MIC dari EEP berada di kisaran 1,21-9,75 g / mL sedangkan MBCS / MFC adalah antara 2,43 dan 19,5 g / mL. Waktu yang diperlukan untuk mencapai 90% (1 log10) penurunan pertumbuhan budaya berkisar antara 1,06 dan 3,53 jam pada nilai-nilai MIC masing-masing. PAE untuk semua budaya adalah> 24 h.

Kesimpulan: India propolis Lebah memiliki sifat kompleks dengan senyawa kimia 24. Memiliki aktivitas antimikroba spektrum luas ampuh. Temuan terakhir, dalam hubungannya dengan sifat bioaktif lainnya, bisa memberikan dasar ilmiah untuk penggunaan populer di obat rakyat India.