MATERIALS AND METHODSAnimals and as

MATERIALS AND METHODS
Animals and aspirin administration
After obtaining the approval of the Institutional
Review Board of our medical
school, all experiments were carried out in accordance
with the Guidelines of the Animal
Care and use ethics committee of Baqiyatallah
University of medical sciences. Thirty pregnant
Albino Wistar rats weighting 230 ± 20 g
were maintained under standard laboratory
conditions. Animals were housed in an environment
of 21±0.5 °C with a relative humidity
of 50±10% and a 12-h light-dark cycle. Food
and water were always available. To determine
the day of estrus, females were daily
placed with sexually vigorous studs for a brief
observation. Estrus was determined by the occurrence
of lordosis in response to the stud's
sexual approach. Estrous females were housed
with Wistar males for approximately 16 h.
The positive mating was considered as day 0
of the pregnancy. The pregnant rats were separated
and were then randomly divided into
six groups (n=6): Group 1 (Control): Fed with
normal diet and water served as control group.
Group 2 (Sham): Fed with normal diet and
given 2 ml distilled water (as a solvent of aspirin)
orally through gavage from days 8 to 20
of pregnancy as the sham group. Group 3
(Dose A): Like sham group and gave 75
mg/kg aspirin (Darou Pakhsh, Iran) through
gavage. Group 4 (Dose B): Like sham group
and gave 100 mg/kg aspirin through gavage.
Group 5 (Dose C): Like sham group and gave
200 mg/kg aspirin through gavage. Group 6
(Dose D): Like sham group and gave 300
mg/kg aspirin through gavage.

Hasil (Bahasa Indonesia) 2:

BAHAN DAN METODE
Hewan dan administrasi aspirin
Setelah memperoleh persetujuan dari Institutional
Review Board of medis kami
sekolah, semua percobaan yang dilakukan sesuai
dengan Pedoman dari Hewan
Perawatan dan menggunakan etika komite Baqiyatallah
Universitas ilmu medis. Tiga puluh hamil
tikus Wistar Albino bobot 230 ± 20 g
dipertahankan di bawah laboratorium standar
kondisi. Hewan bertempat di lingkungan
dari 21 ± 0,5 ° C dengan kelembaban relatif
50 ± 10% dan siklus gelap-terang 12-jam. Makanan
dan air yang selalu tersedia. Untuk menentukan
hari estrus, perempuan yang sehari-hari
ditempatkan dengan kancing yang kuat seksual untuk singkat
observasi. Estrus ditentukan oleh terjadinya
lordosis dalam menanggapi pejantan ini
pendekatan seksual. Betina estrus bertempat
dengan laki-laki Wistar selama kurang lebih 16 jam.
The kawin positif dianggap sebagai hari 0
kehamilan. Tikus-tikus hamil dipisahkan
dan kemudian secara acak dibagi menjadi
enam kelompok (n = 6): Kelompok 1 (kontrol): Fed dengan
diet normal dan air menjabat sebagai kelompok kontrol.
Kelompok 2 (Sham): Fed dengan diet normal dan
diberikan 2 ml air suling (sebagai pelarut aspirin)
secara lisan melalui gavage dari hari 8 sampai 20
kehamilan sebagai kelompok palsu. Kelompok 3
(Dosis A): Kelompok palsu Seperti dan memberi 75
mg / kg aspirin (Darou pakhsh, Iran) melalui
gavage. Kelompok 4 (Dosis B): kelompok sham Seperti
dan memberi 100 mg / kg aspirin melalui gavage.
Grup 5 (Dosis C): kelompok sham Seperti dan memberi
200 mg / kg aspirin melalui gavage. Kelompok 6
(Dosis D): kelompok sham Seperti dan memberi 300
mg / kg aspirin melalui gavage.