which serves as a substrate for yea

which serves as a substrate for yeast (McDonald et al., 1991). Therefore,
inoculants with bacteria that are able to produce the
highest concentrations of acetic or propionic acids are
preferred. Consequently, for our studies in laboratory
silos, the 5 best LAB strains were chosen based on their
ability to produce each one of these acids. Because lactic
acid is primarily responsible for the decrease in pH
values in silage, the 4 best lactic acid–producing strains
were also chosen for the tests in the experimental silos.
Molecular Identification of Selected Bacterial
Strains. The strains selected for evaluation in the experimental
silos were identified by DNA sequencing.
The bacterial cultures were grown under appropriate
conditions on MRS agar plates, and single colonies were
collected with a sterile pipette tip and resuspended in
40 μL of PCR buffer. The suspension was heated for
10 min at 95°C, and 2 μL of the suspension was used
as the DNA template to amplify the full-length 16S
region by PCR. The approximately 1,500-bp fragment
of the 16S rDNA was amplified using forward primer
27f (5