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Diagnostic Methods for WSDAntibodies (Abs)-based test are being tested for the detection of WSSV. Areverse passive latex agglutination assay (RPLA), which detects WSSVfrom stomach tissue homogenate has been proposed as a useful methodfor virus detection on site (Okumura et al., 2004). Histological methodsusing Hematoxiline and Eosine stain (H&E, Lightner, 1996) have beenused as routine diagnostic technique of WSSV by demonstration ofeosinophilic to pale basophilic, intranuclear inclusion bodies inhypertrophied nuclei of the cuticular epithelial cells and connective tissue,and less frequently, in other tissues
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