In this work, the important chromat

In this work, the important chromatographic factors
were selected and optimized by a central composite design experiment. The selection of factors for
optimization was based on preliminary experiments and
prior knowledge from literature, as well as certain
instrumental limitations. For instance, the mobile phase
pH was fixed at 3.0 as this could influence the
stability.From preliminary experiments,the mobile
phase consisting of a methanol and triethylamine buffer
was employed in which the concentration of methanol
content was varied.The mobile phase flow rate could also moderately influence selectivity in the HPLC
analysis. Therefore, the key factors selected for the
optimization process were methanol concentration (A),
buffer molarity (B), and flow rate (C). Table 1 shows the
levels of each factors studied for finding out the optimum
values and responses. As can be seen in this table, the
ranges of each factor used were: methanol concentration
(60–70%), buffer molarity (15–25 mM), and flow rate
(0.7–0.9 ml/min).

Hasil (Bahasa Indonesia) 2:

Dalam karya ini, faktor-faktor kromatografi penting
dipilih dan dioptimalkan oleh eksperimen desain komposit pusat. Pemilihan faktor untuk
optimasi didasarkan pada percobaan awal dan
pengetahuan dari literatur, serta tertentu
keterbatasan instrumental. Misalnya, fase gerak
pH tetap di 3,0 karena hal ini bisa mempengaruhi
percobaan pendahuluan stability.From, ponsel
fase terdiri dari metanol dan trietilamina penyangga
dipekerjakan di mana konsentrasi metanol
konten adalah varied.The laju aliran fase gerak bisa juga cukup mempengaruhi selektivitas dalam HPLC
analisis. Oleh karena itu, faktor kunci yang dipilih untuk
proses optimasi yang konsentrasi metanol (A),
penyangga molaritas (B), dan laju alir (C). Tabel 1 menunjukkan
tingkat masing-masing faktor diteliti untuk mengetahui optimal
nilai-nilai dan tanggapan. Seperti dapat dilihat pada tabel ini,
rentang masing-masing faktor yang digunakan adalah: konsentrasi metanol
(60-70%), penyangga molaritas (15-25 mM), dan laju aliran
(0,7-0,9 ml / menit).