Ligand and oligonucleotides. The pr

Ligand and oligonucleotides. The preQ1 ligand used in this study was generously
provided by George Garcia; preQ1 was synthesized following established methods46
and additionally purified using reverse-phase HPLC as described47. DNA and LNA
oligonucleotides were purchased from Integrated DNA Technologies Inc. (IDT)
and Exiqon, respectively. Fluorophore-labelled DNA and LNA oligonucleotides
were HPLC-purified by the respective manufacturer. The control and anti-SD
probe RNAs were purchased from IDT with a 50 aminohexyl-linker modification
and labelled with Cy5-NHS ester (GE Healthcare) exactly as described19. The
sequences of all oligonucleotides and cloning primers used in this study are listed in
Supplementary Note 4.

Hasil (Bahasa Indonesia) 1:

Ligan dan Oligonukleotida. Ligan preQ1 yang digunakan dalam penelitian ini adalah murah hatidisediakan oleh George Garcia; preQ1 adalah methods46 didirikan disintesis berikutdan Selain itu murni menggunakan HPLC reverse-fase sebagai described47. DNA dan LNAOligonukleotida dibeli dari DNA terintegrasi teknologi Inc (IDT)dan Exiqon, masing-masing. Oligonukleotida DNA berlabel fluorophore dan LNAitu HPLC disucikan oleh masing-masing produsen. Kontrol dan anti-SDprobe RNA yang dibeli dari IDT dengan modifikasi 50 aminohexyl-linkerdan berlabel dengan Cy5-NHS ester (GE Healthcare) persis seperti yang described19. Theurutan semua Oligonukleotida dan primers kloning yang digunakan dalam kajian ini tercantum dalamCatatan tambahan 4.