Preparation of sampleFor determinat

Preparation of sample
For determination of alpha-mangostin content from PLGAmicrospheres,
an appropriate amount of the lyophilized microspheres
were completely dissolved in acetonitrile, followed by precipitation of
the polymer with 30% v/v 0.01 M PBS (pH 7.4). Following centrifugation
at 4000 rpm for 10 min, the supernatant containing the extracted
alpha-mangostin was filtered through 0.2 μm PTFE microfilter
prior to analysis using a corrective and validated RP-HPLC method.
Concentration of the extracted alpha-mangostin was calculated based
on a linear regression equation of the calibration curve.

Hasil (Bahasa Indonesia) 1:

Preparasi sampelUntuk penentuan alpha-mangostin konten dari PLGAmicrospheres,jumlah yang tepat mikrosfer diliposiskanlengkap terlarut dalam asetonitril, diikuti oleh presipitasipolimer dengan 30% v v 0.01 M PBS (pH 7,4). Berikut sentrifugasipada 4000 rpm untuk 10 min, supernatant yang mengandung diekstraksiAlpha-mangostin disaring melalui 0.2 μm PTFE microfiltersebelum analisis metode korektif dan divalidasi KCKT RP.Konsentrasi diekstrak alpha-mangostin dihitung berdasarkanpada persamaan regresi linear kurva kalibrasi.