In this work, the important chromat

In this work, the important chromatographic factors
were selected and optimized by a central composite design experiment. The selection of factors for
optimization was based on preliminary experiments and
prior knowledge from literature, as well as certain
instrumental limitations. For instance, the mobile phase
pH was fixed at 3.0 as this could influence the
stability.13From preliminary experiments,the mobile
phase consisting of a methanol and triethylamine buffer
was employed in which the concentration of methanol
content was varied.The mobile phase flow rate could

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Dalam karya ini, faktor-faktor penting kromatografidipilih dan dioptimalkan oleh percobaan desain komposit pusat. Pemilihan faktoroptimasi didasarkan pada eksperimen Pendahuluan danpengetahuan sebelumnya dari sastra, serta tertentuinstrumental keterbatasan. Sebagai contoh, fase mobilepH ditetapkan pada 3.0 seperti ini dapat mempengaruhiStability.13From awal percobaan, mobilefase yang terdiri dari metanol dan Trietilamina bufferdipekerjakan di mana konsentrasi metanolkonten bervariasi. Laju aliran fase mobile bisa