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A characteristic of enzyme-catalysed reactions, is that for a given enzyme concentration, the initial reaction velocity increases with increasing initial substrate concentration to a limiting value, Vmax.At still higher substrate concentrations, the initial velocity is sometimes found to be less than the maximum value.Substrate inhibition occurs when a molecule of substrate binds to one site on the enzyme and then another molecule of substrate binds to a separate site on enzyme to form a dead-end complexThis can be regarded as a form of uncompetitive inhibition, the extra substrate molecule being the inhibitor.
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