3.1. Plant MaterialRepresentative s

3.1. Plant Material
Representative samples of leaves, stems and roots of Berberis microphylla were collected during the
flowering season at Lago Deseado, Province of Tierra del Fuego (54

22
1
12.4
11
S; 68

45
1
45.0
W) in December
of 2011. A voucher specimen was deposited at the herbarium of the Universidad de Concepcion (Voucher
No CONC 178057). The plant material was vacuum-packed and stored at
20

11
C for further study.
3.2. Alkaloid Extraction
Extraction was performed according to a previously described method [26] with some modifications [6].
In brief, oven-dried and powdered leaves (50 g), stems (50 g) and roots (50 g) of B. microphylla were
sequentially extracted (24, 48 and 72 h) with methanol at roomtemperature. The pooled methanolic extracts
of each plant tissue were evaporated in vacuo at 40

C, and the residues were agitated with 100 mL of
10% HCl for 1 h, incubated for 12 h at 10
(5  80 mL). The CHCl
3

C and then filtered. The filtrates were washed with CHCl
washings yielded brown non-alkaloidal extracts upon evaporation, which were
not investigated. The aqueous phases were adjusted to pH 10 with NH
4
OH and extracted with CHCl
(5  80 mL). The solvent was evaporated to obtain the alkaloid extracts of the roots, stems and leaves.
The alkaloid compositions of the fractions were identical to those previously published [6].
3
3

Hasil (Bahasa Indonesia) 1:

3.1. tanaman bahanSampel perwakilan dari daun, batang dan akar Berberis microphylla dikumpulkan selamamusim berbunga di Lago Deseado, Provinsi Tierra del Fuego (5422112.411S; 6845145.0W) pada bulan Desember2011. Spesimen voucher disimpan di herbarium Universidad de Concepcion (VoucherCONC tidak ada 178057). Bahan tanaman adalah vakum-dikemas dan disimpan di 2011C untuk studi lebih lanjut.3.2. alkaloid ekstraksiEkstraksi dilakukan sesuai dengan metode yang dijelaskan sebelumnya [26] dengan beberapa modifikasi [6].Secara singkat, oven-kering dan bubuk daun (50 g), batang (50 g) dan akar (50 g) B. microphylla yangsecara berurutan diekstraksi (24, 48 dan 72 h) dengan metanol di roomtemperature. Ekstrak methanolic yang terkumpulmasing-masing Jaringan tumbuhan yang menguap di vacuo 40C, dan residu gelisah dengan 100 mL10% untuk 1 h, HCl diinkubasi untuk 12 h 10(5 80 mL). CHCl3C dan kemudian disaring. Diesktraksi itu dicuci dengan CHClpembasuhan menghasilkan ekstrak bebas-alkaloidal coklat berdasarkan penguapan, yangtidak diselidiki. Fase larutan yang disesuaikan pH 10 dengan NH4OH dan diekstraksi dengan CHCl(5 80 mL). Pelarut menguap untuk mendapatkan alkaloid ekstrak akar, batang dan daun.Komposisi alkaloid Fraksi yang identik dengan itu diterbitkan sebelumnya [6].33