In our investigation, out of 126 Ho

In our investigation, out of 126 Holstein bulls, two
bulls were found heterozygous of BLAD (carriers) and
rest were homozygous (normal), as shown in the figure-1.
The percentage of recessive allele in the sample was
calculated to be 1.59. The gene and genotype frequency of
recessive allele was calculated 0.008 and 0.016 in the 126
samples respectively. The size of PCR product was 343bp
and it was subjected to RFLP analysis using Taq-1
restriction enzyme. In normal bulls, the PCR products
yielded two fragments of 191bp and 152bp, whereas
carrier (heterozygous) three fragments of 343bp, 191bp
and 152bp.
In India, Muraleedharan et al. (1999), Patel et al.
(2007), Kumar (2009), Mahdi et al. (2010), and Yathish et
al. (2010), Patel et al. (2010) have reported the carrier
animal’s frequency of 1.33%, 3.23%, 21.82%, 7.31%,
3.64% and 4.76%, in Holstein animals and its crosses
respectively. However one recessive homozygous
(affected) Karan Fries bull was also observed by Yathish
et al. (2010). The incidence of BLAD carriers among top
sires was found to be 23 % in USA (Shuster et al., 1992),
10% in France (Tainturier et al., 1995), 13.5 % in
Germany (Biochard et al., 1995), 2.88 % in Argentina
(Poli et al., 1996), 16 % in Japan (Nagahata et al., 1995),
2.8 % in Brazil (Ribeiro et al., 2000) and 3.33% in Iran
(Norouzy et al., 2005). In our present investigation the
frequency of carrier animals was found as 1.59%.
With the wide use of artificial insemination and
international trading of semen and breeding bulls, these
genetic diseases can spread to large population as
animals’ carrier of the disease look normal. In India,
where HF animals are extensively used for crossbreeding
programmes, it has become necessary to screen all HF and
their crossbreds to minimize the risk of spreading these
diseases among future bulls and bull mothers. However,
continuous screening of young bulls before entering in
artificial insemination (AI) stations is reducing the
incidence of BLAD carriers among HF animals.
As the selection pressure within a breed and AI
programmes are major factors to spread of undesirable
genetic disorders, a routine screening of bulls is required
to reduce the recessive disorder in cattle population.

Hasil (Bahasa Indonesia) 2:

Dalam investigasi kami, dari 126 Holstein lembu jantan, dua
ekor lembu ditemukan heterozigot dari BLAD (operator) dan
sisanya adalah homozigot (normal), seperti yang ditunjukkan pada angka 1 tersebut.
Persentase alel resesif dalam sampel itu
dihitung menjadi 1,59. Gen dan genotipe frekuensi
alel resesif dihitung 0,008 dan 0,016 di 126
sampel masing-masing. Ukuran produk PCR adalah 343bp
dan menjadi sasaran analisis RFLP menggunakan Taq-1
enzim restriksi. Dalam banteng normal, produk PCR
menghasilkan dua fragmen 191bp dan 152bp, sedangkan
pembawa (heterozigot) tiga fragmen 343bp, 191bp
dan 152bp.
Di India, Muraleedharan dkk. (1999), Patel et al.
(2007), Kumar (2009), Mahdi dkk. (2010), dan Yathish et
al. (2010), Patel et al. (2010) telah melaporkan pembawa
frekuensi hewan 1,33%, 3,23%, 21,82%, 7,31%,
3,64% dan 4,76%, di Holstein hewan dan persilangannya
masing-masing. Namun satu resesif homozigot
(terpengaruh) Karan Fries bull juga diamati oleh Yathish
et al. (2010). Insiden operator BLAD antara top
indukan ditemukan 23% di USA (Shuster et al., 1992),
10% di Perancis (Tainturier et al., 1995), 13,5% di
Jerman (Biochard et al., 1995) , 2,88% di Argentina
(Poli et al., 1996), 16% di Jepang (Nagahata et al., 1995),
2,8% di Brazil (Ribeiro et al., 2000) dan 3,33% di Iran
(Norouzy et al., 2005). Dalam penyelidikan kami saat ini
frekuensi hewan pembawa ditemukan sebagai 1,59%.
Dengan luas penggunaan inseminasi buatan dan
perdagangan internasional semen dan lembu peternakan, ini
penyakit genetik dapat menyebar ke populasi yang besar sebagai
pembawa hewan dari penyakit terlihat normal. Di India,
di mana HF hewan secara luas digunakan untuk persilangan
program, telah menjadi perlu untuk menyaring semua HF dan
crossbreds mereka untuk meminimalkan resiko penyebaran ini
penyakit antara bulls masa depan dan ibu banteng. Namun,
skrining terus menerus ekor lembu jantan muda sebelum masuk dalam
inseminasi buatan (AI) stasiun adalah mengurangi
kejadian operator BLAD antara hewan HF.
Sebagai tekanan seleksi dalam berkembang biak dan AI
program faktor utama untuk menyebarkan dari yang tidak diinginkan
kelainan genetik, skrining rutin banteng diperlukan
untuk mengurangi gangguan resesif populasi ternak.