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The present study was the first att
The present study was the first attempt of AI with frozen-thawed
ram semen using a soybean lecithin-based extender, AndroMed, in
Japanese sheep. The commercially available AndroMed (Tiefenbach,
Germany) is a Tris-based buffer containing phospholipids,
citric acid, sugars, antioxidant and glycerol, and has been utilized
for cyropreservation of bovine [7–9] and mountain gazelle semen
[10]. Fresh and frozen ram semen diluted with AndroMed have
been inseminated with satisfactory fertility results in Norway (Paulenz
H.: personal communication). Anel et al. [20] reported that
the soybean lecithin in AndroMed contains a high content of egg
yolk-like phospholipids. Egg yolk is a main component in extenders
for storage and freezing of most mammalian semen including
that of sheep. The active fraction of egg yolk is believed to be a
low-density lipoprotein [1]. However, possible disadvantages ofof microbiological contamination, have also been pointed out [21].
Furthermore, it has been reported that addition of egg yolk reduces
the acrosome integrity and the post-thawing viability of ejaculated
spermatozoa in several species, such as goats [4, 5], rams [1] and
water buffaloes [6]. Our recent study [15] showed that a semen
extender containing10% BSA without egg yolk resulted in acceptable
fertility that is similar to that achieved with extender
containing egg yolk (63 and 67% lambing rates for BSA- and egg
yolk-containing extenders, respectively). Although addition of
BSA demonstrated to develop a synthetic, semi-defined semen
extender in place of egg yolk, complete elimination of egg yolk or
BSA from semen extender and the use of a non-animal derived
compound, such as a soybean lecithin instead is a better choice for
reducing the hygienic risks of contamination of semen doses with
bacteria and mycoplasma (AndroMed [21] or Biociphos plus [22]).
The present AI trial confirmed the fertility results using a BSAcontaining
extender for freezing ram semen and demonstrated that
a completely defined, synthetic semen extender (AndroMed) could
be used for sheep AI with frozen-thawed semen.
ram semen using a soybean lecithin-based extender, AndroMed, in
Japanese sheep. The commercially available AndroMed (Tiefenbach,
Germany) is a Tris-based buffer containing phospholipids,
citric acid, sugars, antioxidant and glycerol, and has been utilized
for cyropreservation of bovine [7–9] and mountain gazelle semen
[10]. Fresh and frozen ram semen diluted with AndroMed have
been inseminated with satisfactory fertility results in Norway (Paulenz
H.: personal communication). Anel et al. [20] reported that
the soybean lecithin in AndroMed contains a high content of egg
yolk-like phospholipids. Egg yolk is a main component in extenders
for storage and freezing of most mammalian semen including
that of sheep. The active fraction of egg yolk is believed to be a
low-density lipoprotein [1]. However, possible disadvantages ofof microbiological contamination, have also been pointed out [21].
Furthermore, it has been reported that addition of egg yolk reduces
the acrosome integrity and the post-thawing viability of ejaculated
spermatozoa in several species, such as goats [4, 5], rams [1] and
water buffaloes [6]. Our recent study [15] showed that a semen
extender containing10% BSA without egg yolk resulted in acceptable
fertility that is similar to that achieved with extender
containing egg yolk (63 and 67% lambing rates for BSA- and egg
yolk-containing extenders, respectively). Although addition of
BSA demonstrated to develop a synthetic, semi-defined semen
extender in place of egg yolk, complete elimination of egg yolk or
BSA from semen extender and the use of a non-animal derived
compound, such as a soybean lecithin instead is a better choice for
reducing the hygienic risks of contamination of semen doses with
bacteria and mycoplasma (AndroMed [21] or Biociphos plus [22]).
The present AI trial confirmed the fertility results using a BSAcontaining
extender for freezing ram semen and demonstrated that
a completely defined, synthetic semen extender (AndroMed) could
be used for sheep AI with frozen-thawed semen.
0/5000
The present study was the first attempt of AI with frozen-thawed
ram semen using a soybean lecithin-based extender, AndroMed, in
Japanese sheep. The commercially available AndroMed (Tiefenbach,
Germany) is a Tris-based buffer containing phospholipids,
citric acid, sugars, antioxidant and glycerol, and has been utilized
for cyropreservation of bovine [7–9] and mountain gazelle semen
[10]. Fresh and frozen ram semen diluted with AndroMed have
been inseminated with satisfactory fertility results in Norway (Paulenz
H.: personal communication). Anel et al. [20] reported that
the soybean lecithin in AndroMed contains a high content of egg
yolk-like phospholipids. Egg yolk is a main component in extenders
for storage and freezing of most mammalian semen including
that of sheep. The active fraction of egg yolk is believed to be a
low-density lipoprotein [1]. However, possible disadvantages ofof microbiological contamination, have also been pointed out [21].
Furthermore, it has been reported that addition of egg yolk reduces
the acrosome integrity and the post-thawing viability of ejaculated
spermatozoa in several species, such as goats [4, 5], rams [1] and
water buffaloes [6]. Our recent study [15] showed that a semen
extender containing10% BSA without egg yolk resulted in acceptable
fertility that is similar to that achieved with extender
containing egg yolk (63 and 67% lambing rates for BSA- and egg
yolk-containing extenders, respectively). Although addition of
BSA demonstrated to develop a synthetic, semi-defined semen
extender in place of egg yolk, complete elimination of egg yolk or
BSA from semen extender and the use of a non-animal derived
compound, such as a soybean lecithin instead is a better choice for
reducing the hygienic risks of contamination of semen doses with
bacteria and mycoplasma (AndroMed [21] or Biociphos plus [22]).
The present AI trial confirmed the fertility results using a BSAcontaining
extender for freezing ram semen and demonstrated that
a completely defined, synthetic semen extender (AndroMed) could
be used for sheep AI with frozen-thawed semen.
ram semen using a soybean lecithin-based extender, AndroMed, in
Japanese sheep. The commercially available AndroMed (Tiefenbach,
Germany) is a Tris-based buffer containing phospholipids,
citric acid, sugars, antioxidant and glycerol, and has been utilized
for cyropreservation of bovine [7–9] and mountain gazelle semen
[10]. Fresh and frozen ram semen diluted with AndroMed have
been inseminated with satisfactory fertility results in Norway (Paulenz
H.: personal communication). Anel et al. [20] reported that
the soybean lecithin in AndroMed contains a high content of egg
yolk-like phospholipids. Egg yolk is a main component in extenders
for storage and freezing of most mammalian semen including
that of sheep. The active fraction of egg yolk is believed to be a
low-density lipoprotein [1]. However, possible disadvantages ofof microbiological contamination, have also been pointed out [21].
Furthermore, it has been reported that addition of egg yolk reduces
the acrosome integrity and the post-thawing viability of ejaculated
spermatozoa in several species, such as goats [4, 5], rams [1] and
water buffaloes [6]. Our recent study [15] showed that a semen
extender containing10% BSA without egg yolk resulted in acceptable
fertility that is similar to that achieved with extender
containing egg yolk (63 and 67% lambing rates for BSA- and egg
yolk-containing extenders, respectively). Although addition of
BSA demonstrated to develop a synthetic, semi-defined semen
extender in place of egg yolk, complete elimination of egg yolk or
BSA from semen extender and the use of a non-animal derived
compound, such as a soybean lecithin instead is a better choice for
reducing the hygienic risks of contamination of semen doses with
bacteria and mycoplasma (AndroMed [21] or Biociphos plus [22]).
The present AI trial confirmed the fertility results using a BSAcontaining
extender for freezing ram semen and demonstrated that
a completely defined, synthetic semen extender (AndroMed) could
be used for sheep AI with frozen-thawed semen.
Sedang diterjemahkan, harap tunggu..
Penelitian ini adalah upaya pertama dari AI dengan beku-dicairkan
semen ram menggunakan extender berbasis lesitin kedelai, AndroMed, di
domba Jepang. Tersedia secara komersial AndroMed (Tiefenbach,
Jerman) adalah penyangga Tris berbasis mengandung fosfolipid,
asam sitrat, gula, antioksidan dan gliserol, dan telah digunakan
untuk cyropreservation dari sapi [7-9] dan gunung semen kijang
[10]. Semen ram segar dan beku diencerkan dengan AndroMed telah
telah diinseminasi dengan hasil yang memuaskan kesuburan di Norwegia (Paulenz
komunikasi pribadi H .:). Anel et al. [20] melaporkan bahwa
pada lesitin kedelai di AndroMed mengandung kandungan telur
kuning seperti fosfolipid. Kuning telur merupakan komponen utama dalam extender
untuk penyimpanan dan pembekuan sebagian semen mamalia termasuk
yang domba. Fraksi aktif kuning telur diyakini menjadi
lipoprotein low-density [1]. Namun, kemungkinan kerugian ofof kontaminasi mikrobiologi, juga telah menunjukkan [21].
Selain itu, telah dilaporkan bahwa penambahan kuning telur mengurangi
integritas akrosom dan kelangsungan hidup pasca-thawing dari ejakulasi
spermatozoa pada beberapa spesies, seperti kambing [4 , 5], domba [1] dan
kerbau [6]. Kami penelitian terbaru [15] menunjukkan bahwa air mani
extender containing10% BSA tanpa kuning telur mengakibatkan diterima
kesuburan yang mirip dengan yang dicapai dengan extender
kuning telur mengandung (63 dan 67% tingkat beranak untuk BSA- dan telur
kuning mengandung extender, masing-masing ). Meskipun penambahan
BSA menunjukkan untuk mengembangkan sintetis, semen semi-didefinisikan
extender di tempat kuning telur, penghapusan lengkap kuning telur atau
BSA dari semen extender dan penggunaan non-hewani
senyawa, seperti lesitin kedelai bukan merupakan pilihan yang lebih baik untuk
mengurangi risiko kontaminasi higienis dosis semen dengan
bakteri dan Mycoplasma (AndroMed [21] atau Biociphos ditambah [22]).
sidang AI yang hadir menegaskan hasil kesuburan menggunakan BSAcontaining
extender untuk pembekuan semen ram dan menunjukkan bahwa
yang benar-benar didefinisikan, semen sintetis extender (AndroMed) bisa
digunakan untuk domba AI dengan semen beku-dicairkan.
semen ram menggunakan extender berbasis lesitin kedelai, AndroMed, di
domba Jepang. Tersedia secara komersial AndroMed (Tiefenbach,
Jerman) adalah penyangga Tris berbasis mengandung fosfolipid,
asam sitrat, gula, antioksidan dan gliserol, dan telah digunakan
untuk cyropreservation dari sapi [7-9] dan gunung semen kijang
[10]. Semen ram segar dan beku diencerkan dengan AndroMed telah
telah diinseminasi dengan hasil yang memuaskan kesuburan di Norwegia (Paulenz
komunikasi pribadi H .:). Anel et al. [20] melaporkan bahwa
pada lesitin kedelai di AndroMed mengandung kandungan telur
kuning seperti fosfolipid. Kuning telur merupakan komponen utama dalam extender
untuk penyimpanan dan pembekuan sebagian semen mamalia termasuk
yang domba. Fraksi aktif kuning telur diyakini menjadi
lipoprotein low-density [1]. Namun, kemungkinan kerugian ofof kontaminasi mikrobiologi, juga telah menunjukkan [21].
Selain itu, telah dilaporkan bahwa penambahan kuning telur mengurangi
integritas akrosom dan kelangsungan hidup pasca-thawing dari ejakulasi
spermatozoa pada beberapa spesies, seperti kambing [4 , 5], domba [1] dan
kerbau [6]. Kami penelitian terbaru [15] menunjukkan bahwa air mani
extender containing10% BSA tanpa kuning telur mengakibatkan diterima
kesuburan yang mirip dengan yang dicapai dengan extender
kuning telur mengandung (63 dan 67% tingkat beranak untuk BSA- dan telur
kuning mengandung extender, masing-masing ). Meskipun penambahan
BSA menunjukkan untuk mengembangkan sintetis, semen semi-didefinisikan
extender di tempat kuning telur, penghapusan lengkap kuning telur atau
BSA dari semen extender dan penggunaan non-hewani
senyawa, seperti lesitin kedelai bukan merupakan pilihan yang lebih baik untuk
mengurangi risiko kontaminasi higienis dosis semen dengan
bakteri dan Mycoplasma (AndroMed [21] atau Biociphos ditambah [22]).
sidang AI yang hadir menegaskan hasil kesuburan menggunakan BSAcontaining
extender untuk pembekuan semen ram dan menunjukkan bahwa
yang benar-benar didefinisikan, semen sintetis extender (AndroMed) bisa
digunakan untuk domba AI dengan semen beku-dicairkan.
Sedang diterjemahkan, harap tunggu..
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