Disc diffusion methodThe antimicrob

Disc diffusion method
The antimicrobial tests were carried out by the disc diffusion
method as described by Berghe and Vlietinck (1991) using a cell
suspension of about 1.5×106 CFU/ml “colony forming units per
milliliter” obtained following a 0.5 McFarland turbidity standard.
The concentration of the suspension was standardized by adjusting
the optical density to 0.1 at 600nm wavelength (SHIMADZU
UV-120-01 spectrophotometer) (Tereschuck et al., 1997). Petri
dishes were filled with Agar Mueller Hinton medium (bacteria)
or Sabouraud dextrose agar (yeasts) and inoculated with the test

0/5000

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Disc diffusion methodThe antimicrobial tests were carried out by the disc diffusionmethod as described by Berghe and Vlietinck (1991) using a cellsuspension of about 1.5×106 CFU/ml “colony forming units permilliliter” obtained following a 0.5 McFarland turbidity standard.The concentration of the suspension was standardized by adjustingthe optical density to 0.1 at 600nm wavelength (SHIMADZUUV-120-01 spectrophotometer) (Tereschuck et al., 1997). Petridishes were filled with Agar Mueller Hinton medium (bacteria)or Sabouraud dextrose agar (yeasts) and inoculated with the test

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Metode disc difusi
Tes antimikroba dilakukan dengan difusi disk
metode seperti yang dijelaskan oleh Berghe dan Vlietinck (1991) menggunakan sel
suspensi dari sekitar 1,5 × 106 CFU / ml "koloni unit per
mililiter" diperoleh setelah 0,5 McFarland kekeruhan standar.
konsentrasi suspensi standar dengan menyesuaikan
kepadatan optik untuk 0,1 pada 600nm panjang gelombang (Shimadzu
UV-120-01 spektrofotometer) (Tereschuck et al., 1997). Petri
hidangan yang diisi dengan media Agar Mueller Hinton (bakteri)
atau Sabouraud dextrose agar (ragi) dan diinokulasi dengan tes

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