- Teks
- Sejarah
Czech J. Food Sci. Vol. 27, 2009, S
Czech J. Food Sci. Vol. 27, 2009, Special Issue 2: S2-75–S2-81
Detection of Transgenic Papaya Lines: Extraction Protocol Optimisation and Verification of DNA Quality by PCR Assay
Jaroslava Ovesná and Jan Hodek
Department of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech Republic
Abstract: Food matrices are controlled for the presence of authorised and unauthorised GMOs (Genetically Modified Organisms). Transgenic papaya was developed and approved for the market in the USA. However, authorisation in EC have not been accomplished. Recently, unauthorised GM papaya was catch on EC market. Therefore it is necessary to dispose by an assay revealing genetically modified fruits. High quality DNA is required for the assay. We tested and optimised CTAB, Wizard and spin column based procedure for DNA extraction. We found that CTAB based proto-col allows isolation of amplifiable DNA from fresh fruit flesh and stones, whereas spin columns alowed isolation of amplifiable DNA from candied fruits. Modified protocol is described. No transgenic fresh fruit or candied fruit from the market was detected.
Detection of Transgenic Papaya Lines: Extraction Protocol Optimisation and Verification of DNA Quality by PCR Assay
Jaroslava Ovesná and Jan Hodek
Department of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech Republic
Abstract: Food matrices are controlled for the presence of authorised and unauthorised GMOs (Genetically Modified Organisms). Transgenic papaya was developed and approved for the market in the USA. However, authorisation in EC have not been accomplished. Recently, unauthorised GM papaya was catch on EC market. Therefore it is necessary to dispose by an assay revealing genetically modified fruits. High quality DNA is required for the assay. We tested and optimised CTAB, Wizard and spin column based procedure for DNA extraction. We found that CTAB based proto-col allows isolation of amplifiable DNA from fresh fruit flesh and stones, whereas spin columns alowed isolation of amplifiable DNA from candied fruits. Modified protocol is described. No transgenic fresh fruit or candied fruit from the market was detected.
0/5000
Czech J. Food Sci. Vol. 27, 2009, Special Issue 2: S2-75–S2-81Detection of Transgenic Papaya Lines: Extraction Protocol Optimisation and Verification of DNA Quality by PCR AssayJaroslava Ovesná and Jan HodekDepartment of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech RepublicAbstract: Food matrices are controlled for the presence of authorised and unauthorised GMOs (Genetically Modified Organisms). Transgenic papaya was developed and approved for the market in the USA. However, authorisation in EC have not been accomplished. Recently, unauthorised GM papaya was catch on EC market. Therefore it is necessary to dispose by an assay revealing genetically modified fruits. High quality DNA is required for the assay. We tested and optimised CTAB, Wizard and spin column based procedure for DNA extraction. We found that CTAB based proto-col allows isolation of amplifiable DNA from fresh fruit flesh and stones, whereas spin columns alowed isolation of amplifiable DNA from candied fruits. Modified protocol is described. No transgenic fresh fruit or candied fruit from the market was detected.
Sedang diterjemahkan, harap tunggu..
Bahasa lainnya
Dukungan alat penerjemahan: Afrikans, Albania, Amhara, Arab, Armenia, Azerbaijan, Bahasa Indonesia, Basque, Belanda, Belarussia, Bengali, Bosnia, Bulgaria, Burma, Cebuano, Ceko, Chichewa, China, Cina Tradisional, Denmark, Deteksi bahasa, Esperanto, Estonia, Farsi, Finlandia, Frisia, Gaelig, Gaelik Skotlandia, Galisia, Georgia, Gujarati, Hausa, Hawaii, Hindi, Hmong, Ibrani, Igbo, Inggris, Islan, Italia, Jawa, Jepang, Jerman, Kannada, Katala, Kazak, Khmer, Kinyarwanda, Kirghiz, Klingon, Korea, Korsika, Kreol Haiti, Kroat, Kurdi, Laos, Latin, Latvia, Lituania, Luksemburg, Magyar, Makedonia, Malagasi, Malayalam, Malta, Maori, Marathi, Melayu, Mongol, Nepal, Norsk, Odia (Oriya), Pashto, Polandia, Portugis, Prancis, Punjabi, Rumania, Rusia, Samoa, Serb, Sesotho, Shona, Sindhi, Sinhala, Slovakia, Slovenia, Somali, Spanyol, Sunda, Swahili, Swensk, Tagalog, Tajik, Tamil, Tatar, Telugu, Thai, Turki, Turkmen, Ukraina, Urdu, Uyghur, Uzbek, Vietnam, Wales, Xhosa, Yiddi, Yoruba, Yunani, Zulu, Bahasa terjemahan.
- Senang bisa kenal sama kamu
- Mereka sedang menyusu botol
- bego
- No liike saxiy
- diam
- there are the mistakes found in the sent
- noise signal is inverted by the unity-ga
- And I know I may end up failing too
- noise signal is inverted by the unity-ga
- diam kamuh
- noise signal is inverted by the unity-ga
- kamu
- dasar
- where Rd(n) and Rx(n) are the autocorrel
- I'm faking u very nice
- We can define the prediction gain of the
- yang
- We can define the prediction gain of the
- there ain't no way
- tanteh
- YANG
- Don't you get job in shalu
- idiot
- demam india
