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The results of material removal for the unhairing/ liming step were similar to the TOC and soluble protein analyses (Figures 3 and 4). As shown in Table 2, test 1 cannot be compared graphically to the other tests because of the dilution of chemicals due to the added water. The coenzymatic processes were more efficient than the chemical methods (tests 3-6 compared to test2). Although the A/B enzymes were applied at a lower concentration than the D/E enzymes, they showed better results (tests 3 and 4). Increasing the concentrations of both the A/B and D/E combinations led to an insignificant increase of organic matter content in the bath. This means that the excess of enzyme does not improve the release of organic matter. The results of the enzymatic activity assays are summarized in Table 3, which presents the average values of the specific activity and the corresponding standard deviation for each characterization. The activities were determined at pH 8 (according to the analytical procedure) and at the pH of the processes (pH 9 for soaking and pH 12 for unhairing/liming) to observe the susceptibility of the enzymes to variable conditions and, in particular, to observe their activity during the hide processing stage. The specific activities (Table 3) were obtained from the measurements of enzymatic activity (U) divided by the mass in grams of enzyme used (as previously described in the methods).
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