3.2. Anti Recombinant OMPw Monoclon

3.2. Anti Recombinant OMPw Monoclonal Antibody

The screening results showed three clones of
monoclonal antibodies against rOMPw. Two out of
three clones, D4F10 and D8D6 produced IgG1 and
clone A7C5C10 produced IgM. Clone D8D6 had
higher OD value (1.80) comparing to D4F11 (1.36)
therefore, in this research we followed up our work
on clone D8D6 which produced IgG1 monoclonal
antibody. The titer of purified antibody of ascitic
is shown in Fig. 2. As shown in Fig. 2 mouse
monoclonal antibody showed very high absorbance
values in ELISA at high dilutions between 1:250 and
1:8000 with recombinant OMPw antigen. The results of
isotype identification of monoclonal antibody against
rOMPw with iso strip kit showed that produced
antibody is IgG2a/ĸ(Fig. 3). The specificity of
monoclonal antibody to rOMPw was also determined
by SDS-PAGE and immuno-blat (Fig. 4 and 5).