he oil and ethanolic extracts were

he oil and ethanolic extracts were dissolved in ethanol to a fi-nal concentration of 40 mg/ml and sterilised by filtration through
0.45 lm Millipore filters. The antibacterial test was then carried
out by agar disc diffusion method (Murray, Baron, Pfaller, Tenover,
& Yolke, 1995) using 100 l l of standardised inoculum suspension
containing 10
7
CFU/ml of bacteria. To prepare standardised inocu-lum, bacteria were grown overnight in LB broth that was main-tained at 37 C with constant agitation until the density matched
the turbidity of a 0.5 McFarland standard. Tube dilution with ster-ile saline was carried out to obtain inocula of 10
7
CFU/ml. Then
sterile filter paper discs (6 mm diameter) were impregnated with
10l l of diluted oil and extracts (corresponding to 400 lg/disc)
separately, using capillary micro-pipette and placed on the inocu-lated LB agar. Negative controls were prepared by soaking the disc
with ethanol only. Standard reference antibiotics, tetracycline and
streptomycin (10lg/disc, each from Sigma-Aldrich Co., St Louis,
MO), were used as positive controls for the tested bacteria. The
plates were incubated at 37C for 24 h. Antibacterial activity was
evaluated by measuring the diameter of the zones of inhibition
against the tested bacteria. Each assay in this experiment was rep-licated three times.

Hasil (Bahasa Indonesia) 1:

Ia minyak dan ekstrak ethanolic larut dalam etanol fi-nal konsentrasi 40 mg/mL dan ia mensterilkan oleh filtrasi melalui0.45 lm Millipore filter. Antibakteri ujian kemudian dilakukanoleh agar-agar disc difusi metode (Murray, Baron, Pfaller, Tenover,& Yolke, 1995) menggunakan 100 l l standar inoculum suspensiyang mengandung 107CFU/ml bakteri. Untuk mempersiapkan inocu-lum standar, bakteri yang ditanam semalam di LB kaldu yang utama-tained di 37 C dengan konstan agitasi sampai kepadatan cocokkekeruhan 0.5 McFarland standar. Tabung pengenceran dengan One Piece-ile saline dilaksanakan untuk mendapatkan inocula 107CFU / ml. kemudiansteril filter paper Disc (diameter 6 mm) yang diresapi dengan10l l diencerkan minyak dan ekstrak (sesuai dengan lg/disk 400)secara terpisah, menggunakan kapiler mikro pipette dan ditempatkan pada agar LB inocu-laranangan dan saksi. Negatif kontrol telah dipersiapkan oleh perendaman diskdengan etanol saja. Referensi standar antibiotik, Tetrasiklin danStreptomisin (10lg/disc, masing-masing dari Co Sigma Aldrich, St Louis,MO), yang digunakan sebagai kontrol positif untuk bakteri diuji. Thepiring diinkubasi di 37 C untuk 24 h. aktivitas antibakteridievaluasi oleh ukuran zona hambatmelawan bakteri diuji. Masing-masing assay dalam percobaan ini adalah rep-licated tiga kali.