EXPERIMENTAL PROCEDURESMaterials—Bo

EXPERIMENTAL PROCEDURES
Materials—Bovine enteropeptidase (BEK)1 purified from intestine was purchased from Biozyme (San Diego, CA). Bovine trypsinogen, bovine trypsin, bovine pancreatic trypsin inhibitor (BPTI), soybean trypsin inhibitor (STI), and trypsin-Sepharose 4B were purchased from Worthington. The enteropeptidase substrate Gly-Asp-Asp-Asp-Asp-Lys-b-naphthylamide (GD4K-NA) was purchased from Bachem (Philadelphia, PA). Chromogenic substrates S-2366 (,Glu-Pro-Arg-p-nitroanilide, where ,Glu is pyroglutamic acid) and S-2765 (Z-D-Arg-Gly-Argp-nitroanilide) were purchased from Chromogenix (Molndal, Sweden).

Hasil (Bahasa Indonesia) 1:

PROSEDUR EKSPERIMENTALBahan — Bovine enteropeptidase (BEK) 1 dimurnikan dari usus dibeli dari Biozyme (San Diego, CA). Trypsinogen sapi, sapi tripsin, inhibitor tripsin pankreas sapi (BPTI), kedelai inhibitor tripsin (IMS) dan 4B tripsin-Sepharose dibeli dari Worthington. Substrat enteropeptidase Gly-Asp-Asp-Asp-Asp-Lys-b-naphthylamide (GD4K-NA) dibeli dari Bachem (Philadelphia, PA). Substrat chromogenic S-2366 (, Glu-Pro-Arg-p-nitroanilide, di mana, Glu adalah asam pyroglutamic) dan S-2765 (Z-D-Arg-Gly-Argp-nitroanilide) itu dibeli dari Chromogenix (Molndal, Swedia).

Hasil (Bahasa Indonesia) 2:

PROSEDUR PERCOBAAN
Bahan-Bovine enteropeptidase (BEK) 1 dimurnikan dari usus dibeli dari Biozyme (San Diego, CA). Tripsinogen sapi, tripsin sapi, tripsin pankreas sapi inhibitor (BPTI), tripsin inhibitor kedelai (STI), dan tripsin-Sepharose 4B yang dibeli dari Worthington. The enteropeptidase substrat Gly-Asp-Asp-Asp-Asp-Lys-b-naphthylamide (GD4K-NA) dibeli dari Bachem (Philadelphia, PA). Substrat kromogenik S-2366 (, Glu-Pro-Arg-p-nitroanilide, di mana, Glu adalah asam pyroglutamic) dan S-2765 (ZD-Arg-Gly-Argp-nitroanilide) yang dibeli dari Chromogenix (Molndal, Swedia).